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Scleral ossicles: angiogenic scaffolds, a novel biomaterial for regenerative medicine applications

机译：Scleral卵石：血管生成支架，一种用于再生医学应用的新型生物材料

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摘要

Given the current prolonged life expectancy, various pathologies affect increasingly the aging subjects. Regarding the musculoskeletal apparatus, bone fragility induces more susceptibility to fractures, often not accompanied by good ability of self-repairing, in particular when critical-size defects (CSD) occur. Currently orthopedic surgery makes use of allografting and autografting which, however, have limitations due to the scarce amount of tissue that can be taken from the donor, the possibility of disease transmission and donor site morbidity. The need to develop new solutions has pushed the field of tissue engineering (TE) research to study new scaffolds to be functionalized in order to obtain constructs capable of promoting tissue regeneration and achieve stable bone recovery over time. This investigation focuses on the most important aspect related to bone tissue regeneration: the angiogenic properties of the scaffold to be used. As an innovative solution, scleral ossicles (SOs), previously characterized as natural, biocompatible and spontaneously decellularized scaffolds used for bone repair, were tested for angiogenic potential and biocompatibility. To reach this purpose, in ovo Chorioallantoic Membrane Assay (CAM) was firstly used to test the angiogenic potential; secondly, in vivo subcutaneous implantation of SOs (in a rat model) was performed in order to assess the biocompatibility and the inflammatory response. Finally, thanks to the analysis of mass spectrometry (LCMSQE), the putative proteins responsible for the SO angiogenic properties were identified. Thus, a novel natural biomaterial is proposed, which is (i) able to induce an angiogenic response in vivo by subcutaneous implantation in a non-immunodeficient animal model, (ii) which does not induce any inflammatory response, and (iii) is useful for regenerative medicine application for the healing of bone CSD.
机译：鉴于目前的延长预期寿命，各种病理学越来越多地影响老化受试者。关于肌肉骨骼装置，骨脆性诱导对裂缝的易感性更易于，通常不伴随自修复的良好能力，特别是当发生临界尺寸缺陷（CSD）时。目前骨科手术利用同种异体和自动化，然而，由于可以从供体中取出的组织量，疾病传播和供体现场发病率的可能性具有局限性。开发新解决方案的需要推动了组织工程（TE）研究的领域，以研究官能化的新支架，以便获得能够促进组织再生的构建体并随着时间的推移实现稳定的骨恢复。本研究侧重于与骨组织再生相关的最重要方面：使用支架的血管生成特性。作为一种创新的解决方案，测试以血管生成潜力和生物相容性进行用于骨骼修复的天然，生物相容性和自发性脱细胞的支架的巩膜粒状（SOS）。为了达到此目的，在ovo血管基脉膜测定（凸轮）中首先用于测试血管生成潜力;其次，在体内皮下植入SOS（在大鼠模型中）以评估生物相容性和炎症反应。最后，由于质谱法（LCMSQE）的分析，鉴定了血管生成特性的调用蛋白质。因此，提出了一种新型天然生物材料，其是（i）能够通过皮下植入在非免疫缺陷动物模型中诱导体内血管生成反应，（ii）不诱导任何炎症反应，（iii）是有用的用于再生医学施用骨CSD的愈合。

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  • 来源
    《Biomaterials Science》 |2020年第1期|共13页
  • 作者

    Checchi Marta; Bertacchini Jessika; Cavani Francesco; Magaro Maria Sara; Reggiani Bonetti Luca; Pugliese Geltrude Rita; Tamma Roberto; Ribatti Domenico; Maurel Delphine B.; Palumbo Carla;

  • 作者单位

    Univ Modena &

    Reggio Emilia Dept Biomed Metab Sci &

    Neurosci I-41125 Modena Italy;

    Univ Modena &

    Reggio Emilia Dept Biomed Metab Sci &

    Neurosci I-41125 Modena Italy;

    Univ Modena &

    Reggio Emilia Dept Biomed Metab Sci &

    Neurosci I-41125 Modena Italy;

    Univ Modena &

    Reggio Emilia Dept Biomed Metab Sci &

    Neurosci I-41125 Modena Italy;

    Univ Modena &

    Reggio Emilia Dept Pathol I-41125 Modena Italy;

    Univ Modena &

    Reggio Emilia Dept Biomed Metab Sci &

    Neurosci I-41125 Modena Italy;

    Univ Bari Med Sch Sect Human Anat &

    Histol Dept Basic Med Sci Neurosci &

    Sensory Organs I-70124 Bari Italy;

    Univ Bari Med Sch Sect Human Anat &

    Histol Dept Basic Med Sci Neurosci &

    Sensory Organs I-70124 Bari Italy;

    Univ Bordeaux Pharmaceut Sci Dept Biotis INSERM Unit 1026 F-33076 Bordeaux France;

    Univ Modena &

    Reggio Emilia Dept Biomed Metab Sci &

    Neurosci I-41125 Modena Italy;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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