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首页> 外文期刊>Biochemistry and Cell Biology >Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis
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Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis

机译：合成牛乳铁蛋白肽对肠寄生虫Giardia Intestinalis的寄生作用

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Giardia intestinalis is the most common infectious protozoan parasite in children. Despite the effectiveness of some drugs, the disease remains a major worldwide problem. Consequently, the search for new treatments is important for disease eradication. Biological molecules with antimicrobial properties represent a promising alternative to combat pathogens. Bovine lactoferrin (bLF) is a key component of the innate host defense system, and its peptides have exhibited strong antimicrobial activity. Based on these properties, we evaluated the parasiticidal activity of these peptides on G. intestinalis . Trophozoites were incubated with different peptide concentrations for different periods of time, and the growth or viability was determined by carboxyfluorescein-succinimidyl-diacetate-ester (CFDA) and propidium iodide (PI) staining. Endocytosis of peptides was investigated by confocal microscopy, damage was analyzed by transmission and scanning electron microscopy, and the type of programmed cell death was analyzed by flow cytometry. Our results showed that the LF peptides had giardicidal activity. The LF peptides interacted with G. intestinalis and exposure to LF peptides correlated with an increase in the granularity and vacuolization of the cytoplasm. Additionally, the formation of pores, extensive membrane disruption, and programmed cell death was observed in trophozoites treated with LF peptides. Our results demonstrate that LF peptides exhibit potent in vitro antigiardial activity.]]>
机译：Giardia Intestinalis是儿童中最常见的传染性原生动物寄生虫。尽管有些药物有效，但该疾病仍然是全球主要问题。因此，对新治疗的寻求对于疾病而言是重要的。具有抗微生物性质的生物分子代表了对抗病原体的有希望的替代品。牛乳铁蛋白（BLF）是先天宿主防御系统的关键组成部分，其肽表现出强烈的抗微生物活性。基于这些性质，我们评估了这些肽对G. Intestinalis的寄生虫活性。在不同的时间段与不同的肽浓度孵育滋养体，并且通过Carboxyfluoresce-琥珀酰亚胺酰亚胺 - 酯（CFDA）和碘化丙烯酸铅（PI）染色来测定生长或活力。通过共聚焦显微镜研究肽的内吞作用，通过透射和扫描电子显微镜分析损伤，通过流式细胞术分析编程细胞死亡的类型。我们的研究结果表明，LF肽具有吉拉尼霉素活性。 LF肽与G.肠和暴露于LF肽与细胞质的粒度和真空效果的增加相关。另外，在用LF肽处理的滋养体中观察到孔隙，广泛的膜破坏和编程细胞死亡。我们的结果表明，LF肽表现出有效的体外抗原激性活性。]]>

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