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首页> 外文期刊>Biochemistry and Cell Biology >Jatropha-6(17),11 E -diene class derivatives induce apoptosis effects in OVCAR-3 and Caov-4 ovarian cancer cell lines via a mitochondrial pathway
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Jatropha-6(17),11 E -diene class derivatives induce apoptosis effects in OVCAR-3 and Caov-4 ovarian cancer cell lines via a mitochondrial pathway

机译：Jatropha-6（17），11e-二烯类衍生物通过线粒体途径诱导卵巢-3和CaoV-4卵巢癌细胞系中的凋亡作用

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摘要

E -diene class derivatives, compounds A, B, and C that were extracted from Euphorbia osyridea Boiss, in the ovarian cancer cell lines Caov-4 and OVCAR-3. The OVCAR-3 and Caov-4 cell lines were treated with different concentrations of these compounds. Cytotoxicity was evaluated using MTT, clonogenic survival assay, and flow cytometry assays. The production of reactive oxygen species (ROS), mitochondrial membrane potential (Δ Ψm ), and the activity of caspase 3 and 9 were evaluated. Compounds A, B, and C reduced cell viability in a dose-dependent manner ( P 50 values were calculated as 46.27 ± 3.86, and 38.81 ± 3.30 μmol/L for compound A, 36.48 ± 3.18 and 42.59 ± 4.50 μmol/L for compound B, and 85.86 ± 6.75 and 75.65 ± 2.56 μmol/L for compound C against the Caov-4 and OVCAR-3 cell lines, respectively. Apoptosis evaluation showed that jatrophane derivatives increase both early and late apoptosis ( P Ψm , and the activity of caspase 3 and 9 in the treated cells. These results showed that compounds A and B have significant inhibitory effects on OVCAR-3 and Caov-4 proliferation and induction of apoptosis.
机译：从卵巢癌细胞系CaOV-4和OVCAR-3中，从大戟属oyysyrea Boiss中提取的E-二烯类衍生物，化合物A，B和C.用不同浓度的这些化合物处理OVCAR-3和CAOV-4细胞系。使用MTT，克隆灭性存活测定和流式细胞术测定评估细胞毒性。评价反应性氧物质（ROS），线粒体膜电位（Δε）的生产和胱天蛋白3和9的活性。化合物A，B和C以剂量依赖的方式降低细胞活力（P 50值计算为化合物A，36.48±3.18和42.59±4.50μmol/ L.化合物的38.81±3.30μmol/ L. B，和85.86±6.75和75.65±2.56μmol/ L分别对CaOV-4和ovcar-3细胞系。凋亡评估表明，服从衍生物早期和晚期细胞凋亡（pψm，和活性处理过的细胞中的胱天蛋白酶3和9.这些结果表明，化合物A和B对OVCAR-3和CAOV-4增殖和诱导细胞凋亡具有显着的抑制作用。

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