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Tumor Necrosis Factor gene polymorphism results in high TNF level in sepsis and septic shock

机译：肿瘤坏死因子基因多态性导致败血症和败血性休克中高TNF水平

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Introduction: Systemic sepsis releases several cytokines among which tumor necrosis factor alfa (TNF??) has emerged as key cytokine causing septic shock. Single Nucleotide Polymorphisms (SNPs) at positions -238, -308, -376 and +489 in the promoter region of TNF gene exhibit differential association to inflammation and increased TNF production in sepsis. Materials and Methods: This research work was carried out in 278 critically ill patients and 115 controls. The patients were divided into four groups: Healthy controls, SIRS, Sepsis and Septic shock. Plasma cytokine level was evaluated by ELISA. Specific sequences of TNF gene (-238, -308, -376, +489) were amplified using polychromase chain reaction (PCR). SNP detected by BamHiI, NcoI, FokI, TaiI restriction enzymes. Results: Mean plasma TNF?? level in healthy Control group was 8.37 ?? 2.23. pg/ml, in SIRS group, the mean plasma TNF?? level was 77.99 ?? 5.51. pg/ml, in Sepsis patients 187.1 ?? 14.33. pg/ml and in septic shock 202.2 ?? 14.85. pg/ml; range 56.17-417.1. pg/ml. SNP was studied among different patient groups, which showed a higher frequency of mutants among sepsis and shock patients as compared to control. Conclusion: Plasma TNF alpha level was significantly high in patients with sepsis and septic shock. SNP of TNF gene showed significant association between polymorphism and development of severe sepsis and septic shock, this would help us in evaluating patients at high risk for septic shock and such patients needed to obtain a rational basis for therapy. ? 2012.
机译：简介：全身性败血症释放几种细胞因子，其中肿瘤坏死因子α（TNFα）已成为引起败血性休克的关键细胞因子。 TNF基因启动子区域中-238，-308，-376和+489处的单核苷酸多态性（SNP）与脓毒症的炎症反应和TNF产生增加相关。材料和方法：这项研究工作是在278名重症患者和115名对照中进行的。将患者分为四组：健康对照组，SIRS，败血症和败血性休克。通过ELISA评估血浆细胞因子水平。使用多色酶链反应（PCR）扩增TNF基因的特定序列（-238，-308，-376，+ 489）。通过BamHiI，NcoI，FokI，TaiI限制酶检测到SNP。结果：平均血浆TNF？健康对照组的血糖水平为8.37 ??。 2.23。 pg / ml，在SIRS组中，平均血浆TNF？等级为77.99 ?? 5.51。 pg / ml，脓毒症患者187.1 ?? 14.33。 pg / ml和败血性休克202.2 ?? 14.85。 pg / ml;范围56.17-417.1。 pg /毫升。在不同患者组中研究了SNP，与对照组相比，脓毒症和休克患者的突变体频率更高。结论：败血症和败血性休克患者血浆TNFα水平明显升高。 TNF基因的SNP显示多态性与严重脓毒症和败血性休克的发展之间存在显着相关性，这将有助于我们评估败血性休克高风险患者，并且这些患者需要获得合理的治疗基础。 ？ 2012。

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