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首页> 外文期刊>Cytokine >Expression analysis and characterization of alternatively spliced transcripts of human IL-7Ralpha chain encoding two truncated receptor proteins in relapsed childhood all.
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Expression analysis and characterization of alternatively spliced transcripts of human IL-7Ralpha chain encoding two truncated receptor proteins in relapsed childhood all.

机译：在复发的儿童期中，人IL-7Ralpha链编码两个截短的受体蛋白的交替剪接转录本的表达分析和表征。

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In the family of cytokines and cytokine receptors, alternative splicing of pre-mRNA is a frequently observed process that generates different protein isoforms from a single genetic locus. The splicing-derived cytokine receptor protein isoforms are mostly soluble receptors or show alterations in their cytoplasmic domain. It is possible that receptor abnormalities or a pathological ratio of different isoforms may contribute to leukaemia by circumventing normal growth factor control or altering the balance of proliferation and differentiation. IL-7 plays a critical role in early stages of both B and T cell maturation. Moreover, it stimulates the expansion of mature T cells including anti-tumour reactive cells as well as a number of T and B cell malignancies underlining its potential importance for deregulated lymphoid proliferation and leukaemogenesis. Here, we present detailed data on the expression of the interleukin 7 receptor alpha chain (IL-7Ralpha) in leukaemic cells from 210 children with acute lymphoblastic leukaemia (ALL) and describe two novel alternatively spliced transcripts of human IL-7Ralpha coding for truncated receptor proteins which are still capable of binding IL-7. IL-7Ralpha mRNA expression was more frequent in more mature pre-B ALL [91% (30/33)] than in common [81% (81/100)] or pro-B ALL [64% (18/28)], or even in T ALL [64% (29/45)]. These results are in concordance with flow cytometric analyses on the proportion of IL-7Ralpha bearing cells among total blast cell population. Our results lead us to assume that splicing derived IL-7Ralpha isoforms play a potential role in modulating IL-7 signal transduction and might be important for the pathogenesis of leukaemia. Copyright 2000 Academic Press.
机译：在细胞因子和细胞因子受体家族中，前mRNA的可变剪接是一个经常观察到的过程，可从单个遗传位点产生不同的蛋白质同工型。剪接衍生的细胞因子受体蛋白同工型大多是可溶性受体或在其胞质结构域中显示出变化。受体异常或不同亚型的病理比率可能通过绕过正常的生长因子控制或改变增殖与分化的平衡而导致白血病。 IL-7在B细胞和T细胞成熟的早期阶段起着至关重要的作用。而且，它刺激包括抗肿瘤反应性细胞在内的成熟T细胞的扩增，以及许多T和B细胞恶性肿瘤，突显了其对于失控的淋巴样增生和白血病生成的潜在重要性。在这里，我们提供有关210名急性淋巴细胞白血病（ALL）儿童白血病细胞中白细胞介素7受体α链（IL-7Ralpha）的表达的详细数据，并描述了编码截短受体的人类IL-7Ralpha的两个新颖的剪接转录本仍然能够结合IL-7的蛋白质。 IL-7Ralpha mRNA表达在更成熟的前B ALL [91％（30/33）]中比在普通[81％（81/100）]或pro-B ALL [64％（18/28）]中更为频繁。 ，甚至是T ALL [64％（29/45）]。这些结果与流式细胞仪分析在总胚细胞总数中携带IL-7Ralpha的细胞的比例一致。我们的结果使我们假设剪接衍生的IL-7Rα亚型在调节IL-7信号转导中起潜在作用，并且可能对白血病的发病机制具有重要意义。版权所有2000学术出版社。

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