Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis

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Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis

机译：用于从人粪便样品中提取DNA的方法验证下游微生物组分析

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Background: A formal method validation for biospecimen processing in the context of accreditation in laboratories and biobanks is lacking. A previously optimized stool processing protocol was validated for fitness-for-purpose for downstream microbiome analysis.

机译：背景：缺乏实验室和生物人物的认证背景下的生物学中的正式方法验证。验证了先前优化的粪便处理协议，用于对下游微生物组分析的适用性。

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《Biopreservation and biobanking》 |2020年第2期|共15页
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正文语种 eng
中图分类细胞生物学;
关键词
biobank; microbiome; fit-for-purpose; validation; stool; 16S;

机译：biobank;microbiome;适合目的;验证;凳子;16s;

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1. Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis [J] . Biopreservation and biobanking . 2020,第2期

机译：用于从人粪便样品中提取DNA的方法验证下游微生物组分析
2. Extraction of human nuclear DNA from feces samples using the QIAamp DNA Stool Mini Kit. [J] . Vandenberg N, van Oorschot RA Journal of forensic sciences. . 2002,第5期

机译：使用QIAamp DNA Stool Mini Kit从粪便样品中提取人核DNA。
3. DNA analysis from stool samples: a fast and reliable method avoiding invasive sampling methods in mouse models of bleeding disorders [J] . Kalippke K, Werwitzke S, von Hornung M, Laboratory Animals . 2009,第4期

机译：从粪便样本中进行DNA分析：一种快速可靠的方法，可避免在出血性疾病小鼠模型中采用侵入式采样方法
4. Human-Microbiome-Relations Extraction Method with Context-Dependent Clustering and Semantic Analysis [C] . Shiori HIKICHI, Shiori SASAKI, Yasushi KIYOKI International Conference on Information Modelling and Knowledge Bases . 2017

机译：具有上下文群体聚类和语义分析的人微生物关系提取方法
5. Part I. Evaluation of silver as a reductive amperometric detector electrode for HPLC and FIA. Part II. Evaluation of supercritical fluid extraction (SFE) as a sample preparation technique. Part III. Development and validation of HPLC methods using supercritical fluid extraction and reversed-phase HPLC with electrochemical detection (LCEC). [D] . Schneiderman, Martin Alan. 1990

机译：第一部分：银作为HPLC和FIA的还原型安培检测器电极的评估。第二部分评价超临界流体萃取（SFE）作为样品制备技术。第三部分使用超临界流体萃取和带电化学检测的反相HPLC（LCEC）的HPLC方法的开发和验证。
6. Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction [O] . Hengameh Mirsepasi, Søren Persson, Carsten Struve, 2014

机译：粪便样品中的微生物多样性取决于DNA提取方法：easyMag DNA提取与QIAamp DNA粪便微型试剂盒提取相比
7. Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction [O] . Hengameh Mirsepasi, Søren Persson, Carsten Struve, 2014

机译：粪便样品中的微生物多样性取决于DNA提取方法：easyMag DNA提取与QIAamp DNA粪便微型试剂盒提取相比
8. Further Development and Validation of DNA-Based Methods for Species of Origin Determination and Human DNA Quantitation in Forensic Casework Specimens [R] . 2005

机译：进一步开发和验证基于DNa的方法，用于法医案例中的原产地测定和人类DNa定量

Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis

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