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首页> 外文期刊>Biopreservation and biobanking >Protective Effects of Cryoprotectants and Lyoprotectants on the Survival of Persipeptide Producing Streptomyces zagrosensis UTMC 1154
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Protective Effects of Cryoprotectants and Lyoprotectants on the Survival of Persipeptide Producing Streptomyces zagrosensis UTMC 1154

机译：冷冻保护剂和冻干药物对抗肽生成的血糖霉菌Zagrosensis UTMC 1154的保护作用1154

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Streptomyces sp. are bacteria recognized as the producers of more than half of the known bioactive compounds. Developing appropriate preservation methods for industrial strains of Streptomyces is necessary, as continuous subculture could have significant negative effects on their characteristics, including their potential to produce secondary metabolites. The effects of two common preservation methods on a bioactive metabolite producer, Streptomyces zagrosensis UTMC 1154, were studied. In the cryopreservation method, glycerol and dimethyl sulfoxide (DMSO) were evaluated as cryoprotectants. Three different suspending fluids including skimmed milk, sucrose+gelatin and Mist. dessicans were compared as the freeze-drying methods. Freeze-dried samples were stored at 4 degrees C for 6 months and at 37 degrees C for 1 and 2 weeks in an accelerated storage stability study, which approximately correspond to storage at 4 degrees C for 10 and 20 years, respectively. Frozen samples were stored at -20 degrees C, -70 degrees C and in the vapor phase of liquid nitrogen for 6 months. Skimmed milk and DMSO were the most efficient protectants for survival and functional maintenance of the strain during the lyophilization and cryopreservation processes (p < 0.05), respectively. The survival rate of S. zagrosensis was 95.0% and 99.3% after 6 months of preservation by using skimmed milk as lyoprotectant and DMSO as the cryoprotectant, respectively. The obtained results showed that cryopreservation is the method of choice for long-term preservation of S. zagrosensis. Cryopreservation also led to only 1%-3% reduction in the biological activity of the strain after 6 months preservation in vapor phase of the liquid nitrogen.
机译：streptomyces sp。细菌是否被认为是已知生物活性化合物的一半以上的生产者。开发适当的保存方法对于链霉菌瘤的工业株是必要的，因为连续的亚培培培养物可能对其特征产生显着的负面影响，包括它们产生次级代谢物的可能性。研究了两种常见保存方法对生物活性代谢产物，Streptomyces Zagrosensis UTMC 1154的影响。在冷冻保存方法中，评价甘油和二甲基磺氧化砜（DMSO）作为冷冻保护剂。三种不同的悬浮液，包括脱脂牛奶，蔗糖+明胶和雾。将Dessicans与冷冻干燥方法进行比较。将冷冻干燥的样品在4℃下储存6个月，在加速储存稳定性研究中以37℃持续1和2周，其分别大致对应于4摄氏度的储存10和20年。将冷冻样品储存在-20℃，-70℃，液氮的气相中储存6个月。脱脂牛奶和DMSO分别是冻干和冷冻保存过程中应变的存活和功能性最有效的保护剂（P <0.05）。通过使用脱脂牛奶作为Lyopropotectant和DMSO作为冷冻保护剂，S. Zagrosensis的存活率分别在保存6个月后为95.0％和99.3％。得到的结果表明，冷冻保存是S. Zagrosensis的长期保存的选择方法。在液氮的气相保存6个月后，冷冻保存也导致菌株的生物活性降低仅为1％-3％。

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