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首页> 外文期刊>Biomedicine & pharmacotherapy =: Biomedecine & pharmacotherapie >Analysis of immunostimulatory activity of polysaccharide extracted from Yu-Ping-Feng in vitro and in vivo
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Analysis of immunostimulatory activity of polysaccharide extracted from Yu-Ping-Feng in vitro and in vivo

机译：在体外和体内从玉平峰提取多糖免疫刺激活性分析

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摘要

As a traditional Chinese multiherbal formula, Yu-Ping-Feng (YPF) is frequently used to treat cold, flu and inflammation-associated diseases. We aimed to evaluate the immunostimulatory effects of polysaccharide isolated from YPF (YPF-PS) in vitro and in vivo. In in vitro experiment, macrophage cell proliferation, phagocytosis rate, cytokine and costimulatory molecule release, T lymphocyte proliferation, cell cycle distribution, CD4(+) and CD8(+) T cell percentages were determined. To investigate the in vivo effects of YPF-PS treatment, different doses YPF-PS were administered to chicken vaccinated against Newcastle disease. The immune organ index, lymphocyte proliferation, antibody titer, cell cycle distribution, and the cell percentage of CD4(+) and CD8(+) were assessed. In vitro results indicated that YPF-PS at 15.62 mu g mL(-1) could increase the LPS-induced macrophage cell proliferation and phagocytosis rate significantly. The levels of cytokine (nitric oxide, tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and interferon beta) and costimulatory molecules (CD80 and CD86) were also considerably enhanced. Moreover, YPF-PS could significantly enhance T lymphocyte proliferation individually or synergistically with phytohemagglutinin. It promoted lymphocyte entry into S and G2/M phases and increased the percentages of CD4(+) and CD8(+) T cells effectively. In addition, in vivo experiments showed that YPF-PS could enhance serum HI antibody titer. The results about T lymphocyte proliferation, cell cycle distribution, CD4(+) and CD8(+) cell percentages in chickens were also confirmed. YPF-PS has efficacious immunomodulatory properties and could be used as a new potential immune stimulator for food and medical purposes. (C) 2017 Published by Elsevier Masson SAS.
机译：作为一种传统的中国偏执，玉平峰（YPF）经常用于治疗感冒，流感和炎症相关的疾病。我们旨在评估多糖在体外和体内分离的多糖从ypf（ypf-ps）中的免疫刺激作用。在体外实验中，测定巨噬细胞增殖，吞噬细胞增殖，细胞因子和共刺激分子释放，T淋巴细胞增殖，细胞周期分布，CD4（+）和CD8（+）T细胞百分比。为了探讨YPF-PS治疗的体内效果，将不同的剂量YPF-PS施用于对新城疫疫苗的鸡。评估免疫器官指数，淋巴细胞增殖，抗体滴度，细胞周期分布和CD4（+）和CD8（+）的细胞百分比。体外结果表明，15.62μgmml（-1）的YPF-PS可以显着提高LPS诱导的巨噬细胞增殖和吞噬作用率。细胞因子（一氧化氮，肿瘤坏死因子-α，白细胞介素-1β，白细胞介素-6和干扰素β）和共刺激分子（CD80和CD86）的水平也大大提高。此外，YPF-PS可以用植物血晶蛋白单独增强T淋巴细胞增殖或协同效应。它促进了淋巴细胞进入s和g2 / m相，从而有效地增加了CD4（+）和CD8（+）T细胞的百分比。此外，在体内实验表明YPF-PS可以增强血清HI抗体滴度。还证实了关于T淋巴细胞增殖，细胞周期分布，CD4（+）和CD8（+）细胞百分比的结果。 YPF-PS具有有效的免疫调节性能，可用作用于食品和医学目的的新潜在的免疫刺激器。 （c）2017年由elestvier Masson SA发布。

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