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首页> 外文期刊>Biochemical Engineering Journal >A novel method of vesicle preparation by simple dilution of bicelle solution
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A novel method of vesicle preparation by simple dilution of bicelle solution

机译：通过简单稀释的散塞溶液制备一种新型囊泡制备方法

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摘要

We prepared phospholipid vesicles in continuous dilution from small phospholipid bilayer bicelles. As vesicles are useful as delivery carriers, it is necessary to develop a method of continuous encapsulation. Bicelles are composed of long-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine in bilayers and short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine as a detergent. Bicelles were diluted in a batch system and a flow system and their membrane properties were compared; sizes were analyzed by dynamic light scattering and membrane properties by fluorescence spectroscopy. In the flow system, the initial bicelles (10 - 20 nm) coalesced by dilution and hydration of the detergent into large vesicles ( > 30 nm). Uptake of methylene blue solution revealed the internal aqueous phase. Transmission electron microscopy confirmed the formation of vesicles by dilution. The membrane fluidity of the vesicles showed an ordered gel phase. The results suggest that bicelles can form vesicles by continuous dilution. This method is suitable for encapsulating materials.
机译：我们在连续稀释的小磷脂双层双胶中制备磷脂囊泡。由于囊泡可用作输送载流子，因此必须开发一种连续封装的方法。散哚由双层和短链1,2-二己酰基-N-甘油-3-磷光啉中的长链1,2-Dimyristoyl-Sn-甘油-3-磷光啉组成为洗涤剂。在分批系统中稀释双柠檬，比较流量系统及其膜性能;通过荧光光谱通过动态光散射和膜特性分析尺寸。在流动系统中，通过稀释和水合将洗涤剂的稀释和水合成大囊泡（> 30nm）聚结。吸收亚甲基蓝溶液显示内部水相。透射电子显微镜通过稀释证实了囊泡的形成。囊泡的膜流动性显示出有序的凝胶相。结果表明，散哚可以通过连续稀释来形成囊泡。该方法适用于封装材料。

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