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首页> 外文期刊>BJOG: an international journal of obstetrics and gynaecology >Understanding and interpreting community sequencing measurements of the vaginal microbiome
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Understanding and interpreting community sequencing measurements of the vaginal microbiome

机译:了解和解释阴道微生物组的群落测序测量

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Community‐wide high‐throughput sequencing has transformed the study of the vaginal microbiome, and clinical applications are on the horizon. Here we outline the three main community sequencing methods: (1) amplicon sequencing, (2) shotgun metagenomic sequencing, and (3) metatranscriptomic sequencing. We discuss the advantages and limitations of community sequencing generally, and the unique strengths and weaknesses of each method. We briefly review the contributions of community sequencing to vaginal microbiome research and practice. We develop suggestions for critically interpreting research results and potential clinical applications based on community sequencing of the vaginal microbiome. Tweetable abstract We review the advantages and limitations of amplicon sequencing, metagenomics, and metatranscriptomics methods for the study of the vaginal microbiome.
机译:社区范围的高通量测序转化了阴道微生物组的研究,临床应用在地平线上。 在这里,我们概述了三个主要的社区测序方法:(1)扩增子测序,(2)霰弹枪偏释序列测序,和(3)MetaTranscriptomic测序。 我们讨论了群落测序的优点和局限性,以及每种方法的独特优点和弱点。 我们简要介绍了社区测序对阴道微生物组研究和实践的贡献。 我们为基于阴道微生物群落的社区测序制定批判性解释研究结果和潜在临床应用的建议。 Twelable Abstract我们审查了扩增子测序,偏见组和MetaTransfriptomics方法的优缺点,用于研究阴道微生物组。

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