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首页> 外文期刊>Cytokine >Correlation of neutrophil and monocyte derived interleukin-1 receptor antagonist and interleukin-8 with colitis severity in the rabbit.
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Correlation of neutrophil and monocyte derived interleukin-1 receptor antagonist and interleukin-8 with colitis severity in the rabbit.

机译：兔中性粒细胞和单核细胞衍生的白介素-1受体拮抗剂和白介素8与结肠炎严重程度的相关性。

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Activated neutrophils and monocytes produce interleukin (IL)-8, a pro-inflammatory chemokine, but also IL-1 receptor antagonist (IL-1ra), which is an anti-inflammatory cytokine. We were interested to see the profiles of IL-8 and IL-1ra in the colonic tissue and in the peripheral blood leukocytes (PBL) during the development of immune complex induced colitis in rabbits. IL-1ra and IL-8 in PBL were measured in 26 rabbits at time 0 h, 24 h, and 48 h after induction of colitis. The colons were removed at 48 h for measuring myeloperoxidase (MPO), ulcer area, IL-1ra and IL-8. Epithelial damage, crypt abscess formation and leukocyte infiltration of the colonic tissue were major features of this colitis model. During the development of colitis, there was an increase in circulating neutrophils and monocytes (P<0.0001), but not lymphocytes. Likewise, elevated amounts of IL-1ra (P=0.0001) and IL-8 (P=0.0219) production by PBL were observed following induction of colitis. Flow cytometry revealed major source of IL-1ra was monocytes, while the main sources of IL-8 were neutrophils and monocytes. There was correlation between MPO and ulcer area (Rs=0.6327, P<0.0001). At 24 h, PBL from MPOHigh group (n=11) showed increased IL-1ra (P=0.027) and IL-8 (P=0.0128) levels vs MPOLow group (n=15). IL-8 production by PBL showed correlation with tissue MPO (Rs=0.4273, P=0.0295). The colitis in this model was associated with an increase in circulating monocytes and neutrophils, which released increased amounts of IL-8 and IL-1ra. Further, IL-8 and IL-1ra showed correlation with the severity of colitis. These observations should significantly further understandings on the role of neutrophils and monocytes in the immunopathogenesis of ulcerative colitis.
机译：激活的嗜中性粒细胞和单核细胞产生促炎性趋化因子白介素（IL）-8，但也产生抗炎细胞因子IL-1受体拮抗剂（IL-1ra）。我们感兴趣地观察了免疫复合物诱发的结肠炎在兔体内结肠组织和外周血白细胞（PBL）中IL-8和IL-1ra的分布。在诱导结肠炎后的0小时，24小时和48小时时，在26只兔子中测量了PBL中的IL-1ra和IL-8。在48小时时取出结肠以测量髓过氧化物酶（MPO），溃疡面积，IL-1ra和IL-8。该结肠炎模型的主要特征是上皮损伤，隐窝脓肿形成和结肠组织白细胞浸润。在结肠炎发展期间，循环中的中性粒细胞和单核细胞增加（P <0.0001），但淋巴细胞没有增加。同样，在诱发结肠炎后，观察到PBL产生的IL-1ra（P = 0.0001）和IL-8（P = 0.0219）数量增加。流式细胞仪显示IL-1ra的主要来源是单核细胞，而IL-8的主要来源是中性粒细胞和单核细胞。 MPO与溃疡面积之间存在相关性（Rs = 0.6327，P <0.0001）。在24小时时，MPOHigh组（n = 11）的PBL与MPOLow组（n = 15）相比，IL-1ra（P = 0.027）和IL-8（P = 0.0128）水平升高。 PBL产生的IL-8与组织MPO相关（Rs ＝ 0.4273，P ＝ 0.0295）。该模型中的结肠炎与循环单核细胞和嗜中性粒细胞增加有关，后者释放出增加量的IL-8和IL-1ra。此外，IL-8和IL-1ra与结肠炎的严重程度相关。这些观察结果应该大大了解中性粒细胞和单核细胞在溃疡性结肠炎的免疫发病机制中的作用。

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