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首页> 外文期刊>Cytokine >Thymic stromal lymphopoietin is expressed and produced by caspase-1/NF-kappaB pathway in mast cells.
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Thymic stromal lymphopoietin is expressed and produced by caspase-1/NF-kappaB pathway in mast cells.

机译：胸腺基质淋巴细胞生成素在肥大细胞中通过caspase-1 / NF-kappaB途径表达和产生。

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Thymic stromal lymphopoietin (TSLP) plays a pivotal role in allergic diseases such as atopic dermatitis, asthma, and chronic obstructive pulmonary disease. Although there are many reports regarding function and regulatory mechanism of TSLP in dendritic cells and/or T cells, the regulatory mechanism of TSLP in mast cells has not been fully elucidated. Here, we describe how TSLP is expressed and produced by inflammatory stimulus in mast cells. TSLP mRNA was expressed by phorbol myristate acetate (PMA) plus A23187 stimulation in HMC-1 cells and reached its peak 5h after PMA plus A23187 stimulation. The expression of TSLP mRNA was inhibited by nuclear factor (NF)-kappaB inhibitor. In addition, NF-kappaB luciferase activity was inhibited by caspase-1 inhibitor, indicating that caspase-1 is an upstream of NF-kappaB in mast cells. Furthermore, caspase-1 inhibitor decreased the expression of TSLP mRNA induced by PMA plus A23187. Finally, TSLP production was inhibited by both caspase-1 inhibitor and NF-kappaB inhibitor. These results provide proof of principle that TSLP can be expressed and produced through caspase-1 and NF-kappaB in mast cells and open new perspectives to pharmacologically manipulate the expression and production of TSLP by molecules acting on the caspase-1 and NF-kappaB pathway.
机译：胸腺基质淋巴细胞生成素（TSLP）在变应性疾病（如特应性皮炎，哮喘和慢性阻塞性肺疾病）中起关键作用。尽管关于树突状细胞和/或T细胞中TSLP的功能和调控机制的报道很多，但尚未完全阐明肥大细胞中TSLP的调控机制。在这里，我们描述了如何在肥大细胞中通过炎症刺激表达和产生TSLP。 TSLP mRNA由佛波肉豆蔻酸酯乙酸酯（PMA）加A23187刺激在HMC-1细胞中表达，并在PMA加A23187刺激后5h达到其峰值。 TSLP mRNA的表达受到核因子（NF）-kappaB抑制剂的抑制。另外，caspase-1抑制剂抑制了NF-κB荧光素酶的活性，这表明caspase-1是肥大细胞中NF-kappaB的上游。此外，caspase-1抑制剂可降低PMA加A23187诱导的TSLP mRNA表达。最后，caspase-1抑制剂和NF-κB抑制剂均抑制TSLP的产生。这些结果提供了原理性证据，表明TSLP可通过caspase-1和NF-kappaB在肥大细胞中表达和产生，并为药理作用于caspase-1和NF-kappaB途径的分子开辟TSLP的表达和产生开辟了新的前景。 。

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