...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytokine >Diminished interleukin 6 (IL-6) production during scarless human fetal wound repair.
【24h】

Diminished interleukin 6 (IL-6) production during scarless human fetal wound repair.

机译：无痕人类胎儿伤口修复过程中白细胞介素6（IL-6）产生减少。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fetal wound healing is characterized by minimal inflammation and scarless repair. IL-6 stimulates inflammation in postnatal wound healing. We hypothesized that fetal skin has a diminished IL-6 response and that exogenous IL-6 will result in scar formation. Human adult or fetal skin was placed subcutaneously in SCID mice and incisionally wounded. Wounds were excised after 4, 12, 24 or 72 h for IL-6 mRNA quantification by RT-PCR. In other grafts, 5 microgram of IL-6 was injected at wounding and then harvested at 7 days for analysis of scar formation. IL-6 production was examined in primary cultures of human fetal or adult dermal fibroblasts incubated for 8 h with 0, 0.1, 1 or 10 ng/ml of PDGF-BB. IL-6 mRNA was detected 4 h after wounding in fetal and adult wounds, but by 12 h there was no IL-6 mRNA in the fetal wounds. Adult wounds had IL-6 mRNA persisting to 72 h. IL-6 administration to fetal wounds resulted in scar formation. Fetal fibroblasts produced less IL-6 protein and mRNA at all points examined (P<0.01 vs adult). Diminished production of inflammatory cytokines such as IL-6 may be responsible for the lack of inflammation seen during fetal wound healing. Diminished inflammation may provide a permissive environment for scarless wound healing. Copyright 2000 Academic Press.
机译：胎儿伤口愈合的特点是炎症最小且修复无疤。 IL-6刺激产后伤口愈合中的炎症。我们假设胎儿​​皮肤的IL-6反应减弱，而外源性IL-6会导致疤痕形成。将成人或胎儿皮肤皮下放置在SCID小鼠中并切开伤口。在4、12、24或72小时后切下伤口，以通过RT-PCR定量IL-6 mRNA。在其他移植物中，在伤口处注射5微克IL-6，然后在7天时收获以分析疤痕的形成。在与0、0.1、1或10 ng / ml PDGF-BB孵育8小时的人类胎儿或成年真皮成纤维细胞的原代培养物中检查了IL-6的产生。胎儿和成人伤口受伤后4小时检测到IL-6 mRNA，但到12小时胎儿伤口中没有IL-6 mRNA。成人伤口的IL-6 mRNA持续至72小时。向胎儿伤口施用IL-6导致疤痕形成。胎儿成纤维细胞在所有检查点均产生较少的IL-6蛋白和mRNA（与成人相比，P <0.01）。炎症细胞因子（如IL-6）的产生减少可能是胎儿伤口愈合过程中缺乏炎症的原因。炎症减轻可以为无疤的伤口愈合提供宽松的环境。版权所有2000学术出版社。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号