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首页> 外文期刊>Cytokine >IL-33 is expressed in human osteoblasts, but has no direct effect on bone remodeling.
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IL-33 is expressed in human osteoblasts, but has no direct effect on bone remodeling.

机译：IL-33在人成骨细胞中表达，但对骨重塑没有直接影响。

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摘要

The aim of the present study was to investigate the potential role of the recently discovered IL-1 family member IL-33 in bone remodeling. Our results indicate that IL-33 mRNA is expressed in osteocytes in non-inflammatory human bone. Moreover, IL-33 levels are increased by TNF-alpha and IL-1beta in human bone marrow stromal cells, osteoblasts and adipocytes obtained from three healthy donors. Experiments with the inhibitor GW-9662 suggested that expression of IL-33, in contrast to that of IL-1beta, is not repressed by PPARgamma likely explaining why IL-33, but not IL-1beta, is expressed in adipocytes. The IL-33 receptor ST2L is not constitutively expressed in human bone marrow stromal cells, osteoblasts or CD14-positive monocytes, and IL-33 has no effect on these cells. In addition, although ST2L mRNA is induced by TNF-alpha and IL-1beta in bone marrow stromal cells, IL-33 has the same effects as TNF-alpha and IL-1beta, and, therefore, the biological activity of IL-33 may be redundant in this system. In agreement with this hypothesis, MC3T3-E1 osteoblast-like cells constitutively express ST2L mRNA, and IL-33 and TNF-alpha/IL-1beta similarly decrease osteocalcin RNA levels in these cells. In conclusion, our results suggest that IL-33 has no direct effects on normal bone remodeling.
机译：本研究的目的是调查最近发现的IL-1家族成员IL-33在骨重塑中的潜在作用。我们的结果表明，IL-33 mRNA在非炎症性人骨的骨细胞中表达。此外，在从三个健康供体获得的人骨髓基质细胞，成骨细胞和脂肪细胞中，TNF-α和IL-1beta可提高IL-33水平。用抑制剂GW-9662进行的实验表明，与IL-1beta相比，IL-33的表达不受PPARgamma抑制，这可能解释了为何在脂肪细胞中表达IL-33而不是IL-1beta的原因。 IL-33受体ST2L在人骨髓基质细胞，成骨细胞或CD14阳性单核细胞中不组成性表达，而IL-33对这些细胞没有作用。此外，尽管在骨髓基质细胞中ST2L mRNA被TNF-alpha和IL-1beta诱导，但是IL-33具有与TNF-alpha和IL-1beta相同的作用，因此，IL-33的生物学活性可能在这个系统中是多余的。与此假设相符，MC3T3-E1成骨细胞样细胞组成性表达ST2L mRNA，而IL-33和TNF-α/ IL-1beta同样降低了这些细胞中的骨钙素RNA水平。总之，我们的结果表明IL-33对正常的骨骼重塑没有直接影响。

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