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首页> 外文期刊>Cytokine >Hematological and acute-phase responses to diet-induced obesity in IL-6 KO mice.
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Hematological and acute-phase responses to diet-induced obesity in IL-6 KO mice.

机译：对IL-6 KO小鼠饮食引起的肥胖的血液学和急性期反应。

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Obesity is associated with chronic inflammation and elevated levels of IL-6. The role of IL-6 in induction of acute-phase proteins and modulation of hematological responses has been demonstrated in models of inflammation and aging, but not in obesity. We hypothesized that IL-6 is necessary to regulate the acute-phase response and hematological changes associated with diet-induced obesity (DIO) in mice. Feeding a 60%kcal/fat diet for 13 weeks to C57BL6 WT male mice induced a significant increase in IL-6 expression in visceral adipose tissue (VAT), but not liver, compared to mice fed chow diet. Significantly elevated IL-6 levels were present in the peritoneal lavage fluid, but not plasma, of DIO compared to lean mice. A comparable degree of obesity, hepatomegaly, hyperleptinemia, VAT inflammation and insulin resistance was observed in DIO WT and IL-6 KO mice compared to WT and KO mice fed chow diet. Significant leukocytosis was observed in DIO WT but not DIO KO mice compared to lean groups. A significant reduction in platelet counts, without alterations in platelet size, percentage of circulating reticulated platelets and number of bone marrow megakaryocytes, was present in DIO KO mice compared to each other group. Hepatic expression of thrombopoietin was comparable in each group, with DIO WT and KO mice having reduced VAT expression compared to lean mice. Lean KO mice had significantly elevated plasma levels of thrombopoietin compared to each other group, whereas liver-associated thrombopoietin levels were comparable in each group. Deficiency of IL-6 resulted in blunted hepatic induction of the acute-phase protein serum amyloid A-1, whereas expression of hepcidin-1 and -2, LPS-binding protein, ceruloplasmin, plasminogen activator inhibitor-1 and thrombospondin-1 was IL-6-independent. In conclusion, in the absence of overt metabolic alterations, IL-6 modulates leukocytosis, thrombopoiesis and induction of SAA-1, but not other acute-phase proteins in obese mice.
机译：肥胖与慢性炎症和IL-6水平升高有关。在炎症和衰老模型中已经证明了IL-6在诱导急性期蛋白和调节血液反应中的作用，但在肥胖症中却没有。我们假设IL-6是调节与饮食诱发的肥胖（DIO）相关的小鼠的急性期反应和血液学改变所必需的。与饲喂低脂饮食的小鼠相比，向C57BL6 WT雄性小鼠饲喂60％kcal / f的脂肪饮食13周可诱导内脏脂肪组织（VAT）的IL-6表达显着增加，而肝脏则没有。与瘦小鼠相比，DIO的腹腔灌洗液中IL-6水平显着升高，但血浆中没有。与喂普通饮食的WT和KO小鼠相比，在DIO WT和IL-6 KO小鼠中观察到了相似程度的肥胖，肝肿大，高瘦素血症，VAT炎症和胰岛素抵抗。与瘦肉组相比，在DIO WT中观察到明显的白细胞增多，但在DIO KO小鼠中未观察到。与其他组相比，DIO KO小鼠的血小板计数显着减少，而血小板大小，循环网状血小板百分比和骨髓巨核细胞数量却没有改变。在每组中，血小板生成素的肝表达是相当的，与瘦小鼠相比，DIO WT和KO小鼠的VAT表达降低。与其他各组相比，瘦KO小鼠的血浆血小板生成素水平显着升高，而与肝脏相关的血小板生成素水平在各组中相当。 IL-6缺乏导致急性期蛋白血清淀粉样蛋白A-1的肝诱导减弱，而hepcidin-1和-2，LPS结合蛋白，铜蓝蛋白，纤溶酶原激活物抑制剂-1和血小板反应蛋白1的表达为IL -6独立。总之，在没有明显的代谢改变的情况下，IL-6调节肥胖小鼠的白细胞增多，血小板生成和SAA-1的诱导，但不调节其他急性期蛋白。

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