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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray diffraction analysis of human importin beta-Snail zinc finger domain complex
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Crystallization and preliminary X-ray diffraction analysis of human importin beta-Snail zinc finger domain complex

机译：人importinβ-Snail锌指结构域复合物的结晶和初步X射线衍射分析

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摘要

Snail is a C2H2-type zinc finger transcriptional repressor that induces epithelial-mesenchymal transition by repression of E-cadherin expression levels during embryonic development and tumour progression. Snail is imported into the nucleus by importin beta through direct binding with its four zinc finger domain. The complex between importin beta and Snail four zinc finger domain was crystallized in order to understand the nuclear transport mechanism of Snail. The constituents of the complex were separately expressed and were then co-purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 228.2, b = 77.5, c = 72.0 angstrom, beta = 100.9 degrees and diffracted to 2.5 angstrom resolution.
机译：蜗牛是一种C2H2型锌指转录阻遏物，可通过抑制胚胎发育和肿瘤进展过程中E-钙黏着蛋白的表达水平来诱导上皮-间质转化。蜗牛通过与其四锌指结构域直接结合而由importinβ导入核内。为了了解Snail的核转运机制，importin beta和Snail四个锌指结构域之间的复合物被结晶。分别表达复合物的成分，然后通过悬滴蒸气扩散法进行共纯化和结晶。晶体属于空间群C2，单位晶胞参数a = 228.2，b = 77.5，c = 72.0埃，β= 100.9度，并衍射至2.5埃分辨率。

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