Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii

Yokoyama Hiroshi; Yamashita Takahiro; Horikoshi Naoki; Kurumizaka Hitoshi; Kagawa Wataru

首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii

【24h】

Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii

机译：钙化纤维素分解细菌bescii分泌蛋白Athe_0614的结晶和初步X射线衍射分析

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The Athe_0614 protein is a component of the extracellular proteins secreted by the anaerobic, extremely thermophilic and cellulolytic bacterium Caldicellulosiruptor bescii. The recombinant protein was expressed in Escherichia coli, purified to near-homogeneity and crystallized using polyethylene glycol 2000 monomethyl ether as a precipitant. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 48.4, b = 42.2, c = 97.8 angstrom, beta, = 96.1 degrees, and diffracted to 2.7 angstrom resolution using synchrotron radiation.

机译：Athe_0614蛋白是厌氧，极热和纤维素分解细菌Caldicellulosiruptor bescii分泌的细胞外蛋白的组成部分。重组蛋白在大肠杆菌中表达，纯化至接近均一，并使用聚乙二醇2000单甲醚作为沉淀剂进行结晶。晶体属于单斜晶空间群P2（1），其晶胞参数a = 48.4，b = 42.2，c = 97.8埃，β= 96.1度，并使用同步加速器辐射衍射至2.7埃分辨率。

著录项

来源
《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第4期|共3页
作者
Yokoyama Hiroshi; Yamashita Takahiro; Horikoshi Naoki; Kurumizaka Hitoshi; Kagawa Wataru;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类晶体学;
关键词

相似文献

外文文献
中文文献
专利

1. Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii [J] . Yokoyama Hiroshi, Yamashita Takahiro, Horikoshi Naoki, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第4期

机译：钙化纤维素分解细菌bescii分泌蛋白Athe_0614的结晶和初步X射线衍射分析
2. Protein purification, crystallization and preliminary X-ray diffraction analysis of L-arabinose isomerase from Lactobacillus fermentum CGMCC2921 [J] . Xu Zheng, Li Sha, Liang Jinfeng, Acta crystallographica. Section F, Structural biology communications . 2015,第1期

机译：发酵乳杆菌CGMCC2921的L-阿拉伯糖异构酶的蛋白纯化，结晶及X射线初步分析
3. Expression, purification, crystallization and preliminary X-ray diffraction analysis of the effector-interaction domain of the resistance protein RGA5-A from Oryza sativa L. japonica [J] . Huang Dan, Zhang Yanan, Zhao Yanxiang, Acta crystallographica. Section F, Structural biology communications . 2015,第2期

机译：水稻抗性蛋白RGA5-A的效应子-相互作用域的表达，纯化，结晶和X射线初步分析
4. Preliminary study on extracellular glycoproteins secreted by yeast strain SH_2 [C] . Cheng Zhi, Sun Guopin, Wang SHujun, Proceedings of the Fourth China-Jpan international congress of mycology . 1998

机译：酵母菌株SH_2分泌的胞外糖蛋白的初步研究
5. Stringent starvation protein A: Expression, purification, characterization, and preliminary x-ray diffraction studies [D] . Andrykovitch, Michelle 2002

机译：严格的饥饿蛋白A：表达，纯化，表征和初步X射线衍射研究
6. Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii [O] . Hiroshi Yokoyama, Takahiro Yamashita, Naoki Horikoshi, 2013

机译：钙化纤维素分解细菌bescii分泌蛋白Athe_0614的结晶和初步X射线衍射分析
7. Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa [O] . Bahl, Christopher D., MacEachran, Daniel P., O’Toole, George A., 2009

机译：铜绿假单胞菌分泌的毒力因子Cif的纯化，结晶和初步X射线衍射分析

Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii

摘要

著录项

相似文献

相关主题

期刊订阅