Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

Furuyama Takamitsu; Ishii Yoshikazu; Ohya Norimasa; Tateda Kazuhiro; Hanson Nancy D.; Shimizu-Ibuka Akiko

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Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

机译：铜绿假单胞菌B类碳青霉烯酶IMP-18的纯化，结晶和初步X射线分析

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Class B beta-lactamases are known as metallo-beta-lactamases (MBLs) and they hydrolyze most beta-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4 angstrom -resolution and belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 120.77, c = 96.54 angstrom, alpha = beta = gamma = 90 degrees, suggesting the presence of two molecules in the asymmetric unit.

机译：B类β-内酰胺酶称为金属β-内酰胺酶（MBL），它们可水解大多数β-内酰胺，包括碳青霉烯。 IMP-18是从铜绿假单胞菌克隆的MBL，通过气相扩散过表达，纯化和结晶，用于X射线晶体学分析。初步的X射线分析表明，晶体衍射到2.4埃分辨率，并属于四边形空间群P4（1）2（1）2，其晶胞参数a = b = 120.77，c = 96.54埃，α= β=伽马= 90度，表明在不对称单元中存在两个分子。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第12期|共4页
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Furuyama Takamitsu; Ishii Yoshikazu; Ohya Norimasa; Tateda Kazuhiro; Hanson Nancy D.; Shimizu-Ibuka Akiko;
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1. Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa [J] . Furuyama Takamitsu, Ishii Yoshikazu, Ohya Norimasa, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第12期

机译：铜绿假单胞菌B类碳青霉烯酶IMP-18的纯化，结晶和初步X射线分析
2. Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa [J] . Christopher D. Bahl Daniel P. MacEachran George A. OToole Dean R. Madden Acta Crystallographica Section F . 2010,第1期

机译：铜绿假单胞菌分泌的毒力因子Cif的纯化，结晶和初步X射线衍射分析
3. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX [J] . Joel T. Weadge Patrick P. Yip Howard Robinson Krista Arnett Peter A. Tipton P. Lynne Howell Acta Crystallographica Section F . 2010,第5期

机译：铜绿假单胞菌AlgX的表达，纯化，结晶和初步X射线分析
4. OprD alterations in non-carbapenemase producing Pseudomonas aeruginosa strains [C] . E. Alcaide, M.D. Blasco, M.J. Giménez, International Conference on Environmental, Industrial and Applied Microbiology . 2014

机译：非碳结甲酶生产假单胞菌铜绿假单胞菌菌株的OPRD改变
5. Expression, purification, crystallization and preliminary X-ray analysis of PR-AMP cyclohydrolase, a zinc binding enzyme from Methanobacterium thermoautotrophicum. [D] . Boju, Lorena. 2004

机译：PR-AMP环水解酶的表达，纯化，结晶和初步X射线分析，PR-AMP环水解酶是一种来自嗜热自养甲烷杆菌的锌结合酶。
6. Purification crystallization and preliminary X-ray analysis of IMP-18 a class B carbapenemase from Pseudomonas aeruginosa [O] . Takamitsu Furuyama, Yoshikazu Ishii, Norimasa Ohya, 2013

机译：铜绿假单胞菌B类碳青霉烯酶IMP-18的纯化结晶和初步X射线分析
7. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX [O] . Weadge, Joel T., Yip, Patrick P., Robinson, Howard, 2010

机译：铜绿假单胞菌AlgX的表达，纯化，结晶和初步X射线分析

Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

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