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首页> 外文期刊>Biotechnology Progress >Resveratrol addition to Chinese hamster ovary cell culture media: The effect on cell growth, monoclonal antibody synthesis, and its chemical modification
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Resveratrol addition to Chinese hamster ovary cell culture media: The effect on cell growth, monoclonal antibody synthesis, and its chemical modification

机译：中国仓鼠卵巢细胞培养基的白藜芦醇添加：对细胞生长，单克隆抗体合成的影响及其化学改性

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摘要

The effect of the addition of resveratrol to cell culture media during the production of monoclonal antibodies was investigated. Treatments of Chinese hamster ovary (CHO) cells expressing immunoglobulin G (IgG) with 25 and 50 mu M resveratrol showed that resveratrol was capable of slowing cell growth while almost doubling cell-specific productivity to 4.7 +/- 0.6 pg IgG/cell center dot day, resulting in up to a 1.37-fold increase of the final IgG titer. A resveratrol concentration of 50 mu M slowed the progression through the cell cycle temporarily by trapping cells in the S-phase. Cation exchange chromatography showed no significant difference in the composition of acidic or basic IgG species and size exclusion chromatography indicated no change in fragmentation or aggregation of the recombinant IgG in the treatment groups. Resveratrol could be used as a chemical additive to CHO media where it would enhance IgG productivity and provide a degree of protection against hydroxyl and superoxide free radicals, expanding the range of options for process improvement available to monoclonal antibody manufacturers.
机译：研究了在单克隆抗体期间加入白藜芦醇对细胞培养基的影响。用25和50μmMerveratrol表达免疫球蛋白G（IgG）的中国仓鼠卵巢（Cho）细胞的治疗表明，白藜芦醇能够减缓细胞生长，同时几乎将细胞特异性生产率加倍至4.7 +/- 0.6 pg IgG / Cell中心点日，导致最终IgG滴度的增长率高达1.37倍。通过在S相中捕获细胞，将50μm的白藜芦醇浓度暂时减缓了通过细胞周期的进展。阳离子交换色谱显示在酸性或碱性IgG物种的组合物中没有显着差异，并且尺寸排阻色谱表明治疗组中重组IgG的破碎或聚集的变化没有变化。白藜芦醇可以用作CHO培养基的化学添加剂，在那里它将增强IgG生产力并提供对羟基和超氧化物自由基的保护程度，扩大到单克隆抗体制造商可用于工艺改善的选项范围。

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