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β‐Mannanase production and kinetic modeling from carob extract by using recombinant Aspergillus sojae

机译：通过使用重组曲霉菌Sojae从Carob提取物中产生和动力学建模

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Abstract > The main objectives of this study were to optimize β‐mannanase fermentation conditions by using Response Surface Methodology (RSM) and to model kinetically using the kinetic models. Based on the results, the optimum fermentation conditions were found to be initial sugar concentration of 10°Bx, whey concentration of 0.75% [w/v], and inoculum size of 8% (v/v). Under optimized conditions, β‐mannanase activity ( P ), sugar consumed (Δ S ), maximum β‐mannanase production rate ( Q <sub>P</sub> ), and sugar utilization yield (SUY) were 687.89?U/mL, 47.38?g/L, 118.54?U?mL –1 ?day –1 , and 69.73%, respectively. Kinetic models were employed to describe the optimum β‐mannanase fermentation process. The kinetic analysis of β‐mannanase fermentation showed that β‐mannanase fermentation is growth associated because the α value (U/mgX) is approximately 330‐fold higher than the β value (U/mgX·hr). Nevertheless, maintenance value ( Z ) was lower than γ value, thus showing that Aspergillus niger mainly utilizes the sugars for β‐mannanase production and fungal growth. Consequently, carob extract and whey powder could be used to be cost‐effective carbon and organic nitrogen sources, respectively. It was clearly indicated that the suggested kinetic models can successfully describe the fungal growth, β‐mannanase production, and substrate consumption. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 本研究的主要目的是通过使用响应面方法（RSM）来优化β-甘露烷酶发酵条件，并使用动力学模型来设计。基于该结果，发现最佳发酵条件是初始糖浓度为10°Bx，乳清浓度为0.75％[w / v]，接种物尺寸为8％（v / v）。在优化条件下，β-甘露酶活性（ p </ i> ），消耗糖（δ s </ i> ），最大β-甘露酶的生产率（ q </ i> <sub> p </ sub> ）和糖利用率（Suy）为687.89？U / ml，47.38〜11，118.54？U？ml -1 </ sup> ？日 -1 </ sup> 分别为69.73％。动力学模型用于描述最佳的β-甘露烷酶发酵过程。 β-甘露烷酶发酵的动力学分析表明，β-甘露酶发酵是增长的，因为 α</ i> 价值（U / MGX）比高于330倍 β</ i> 价值（U / MGX·HR）。然而，维护价值（ z </ i> ）低于 γ</ i> 价值，从而表明 曲霉尼日尔</ i> 主要利用β-甘露糖酶生产和真菌生长的糖。因此，CaroB提取物和乳清粉可用于分别是具有成本有效的碳和有机氮源。清楚地表明，建议的动力学模型可以成功描述真菌生长，β-甘露糖酶生产和底物消耗。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Karahalil Ercan&option=202" target="_blank" rel="nofollow">Karahalil Ercan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Germe? Mustafa&option=202" target="_blank" rel="nofollow">Germe? Mustafa;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Turhan Irfan&option=202" target="_blank" rel="nofollow">Turhan Irfan;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Food EngineeringAkdeniz UniversityAntalya Turkey;</p> <p>Department of Food EngineeringAkdeniz UniversityAntalya Turkey;</p> <p>Department of Food EngineeringAkdeniz UniversityAntalya Turkey;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=carob extract&option=203" rel="nofollow">carob extract;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fungal enzyme production&option=203" rel="nofollow">fungal enzyme production;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=kinetic modeling&option=203" rel="nofollow">kinetic modeling;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=optimization&option=203" rel="nofollow">optimization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=whey&option=203" rel="nofollow">whey;</a> </p> <div class="translation"> 机译：CAROB提取物;真菌酶生产;动力学建模;优化;乳清; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021880183.html">β‐Mannanase production and kinetic modeling from carob extract by using recombinant Aspergillus sojae</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Karahalil Ercan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Karahalil Ercan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Germe? 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第十届全国抗生素学术会议 </a> <span> <span> . 2005</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315086651.html">米曲霉菌株筛选及双菌株制曲在酱醪发酵中的应用</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=颜喆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 颜喆</a> <span> . 2016</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120113860003.html">能够仅使用二氧化碳和甲酸生长的重组微生物及使用所述重组微生物产生有用物质的方法</a> <b>[P]</b> . <span> 中国专利： CN113930374A </span> <span> . 2022-01-14</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061201180138.html">特香型大曲制曲工艺中防止青霉菌污染的方法</a> <b>[P]</b> . <span> 中国专利： CN102559449B </span> <span> . 2013.05.01</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130446856877.html">Recombinant Aspergillus sojae, methods for introducing a nucleic acid sequence in Aspergillus sojae, for selecting Aspergillus sojae processed or transfectado,To produce recombinant Aspergillus sojae, Aspergillus sojae mutant or recombinant expression of recombinant fungus processes a sequence of nucleic acid introduced.For the expression of a proteu00ecna or polipeptu00ecdeo, for the production of a proteu00ecna or polipeptu00ecdeo, and to produce a phytase or a proteu00ecna having phytase activity</a> <b>[P]</b> . <span> 外国专利： <!-- --> BR0012856A </span> <span> . 2002-06-11</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：重组酱油曲霉，在酱油曲霉中引入核酸序列，选择酱油曲霉加工的或转染的方法，生产重组酱油曲霉，酱油曲霉突变体或重组真菌的重组表达的方法是引入核酸序列。蛋白或脂蛋白，用于产生蛋白或脂蛋白，并产生肌醇六磷酸酶或具有肌醇六磷酸酶活性的蛋白 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130432778937.html">USE OF ASPERGILLUS SOJAE AS HOST FOR 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