Cloning and functional analysis of farnesyl pyrophosphate synthase (FPPS) gene from <i xmlns='http://www.wiley.com/namespaces/wiley'>Mylabris cichoriiMylabris cichorii

Zha Shenfang; Yin Youping; Wang Yu; Huang Yi; Li Yan; Wang Zhongkang

摘要

Abstract > Cantharidin, a defensive terpene compound synthesized by the meloid beetle (Coleoptera, Meloidae), is an important anticancer agent. However, there has been little study done on how this compound synthesized by the beetle. In this paper, a farnesyl pyrophosphate synthase (FPPS) gene, designated McFPPS , was isolated from Mylabris cichorii by reverse transcription PCR based on conserved domains in other organisms. Multiple alignment analysis showed that the deduced amino acids shared 70% homology with FPPSs from other species and contained typically seven conservative regions. Gene expression profile analysis revealed that McFPPS was expressed throughout the tested growth stages of M. cichorii adults, whereas the transcripts accumulated to the highest level at 20 days in male adults while the highest expression level appeared at 15 days in females. Tissue expression pattern analysis showed that McFPPS was expressed constitutively in all tested tissues and a relatively higher expression level in the alimentary canal of males, but no significant tissue difference in the females. For the first time, a RNA interference strategy was employed to induce a greater suppression of McFPPS mRNA, and thus a sharp decrease in the expression levels of downstream genes and the concentration of product. All these results indicated that McFPPS may be directly involved or play an essential role in the biosynthesis of cantharidin. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> > cantharidin，由甲状腺甲虫（鞘翅目，Meloidake）合成的防御萜烯化合物是重要的抗癌剂。然而，关于如何由甲虫合成的化合物如何完成研究。本文通过逆转录PCR在其他生物中的保守域中分离出焦磷酸合酶（FPPS）基因，从 mylabris cichorii-/ i>中分离出来。多次对准分析表明，推导的氨基酸共享，与其他物种的FPPS同源物质，并且含有七个保守区域。基因表达谱分析显示，在整个测试的生长阶段中表达 MCFPPS </ i>。 CICHORII </ I>成人，而成绩单在男性成年人的20天内累积在最高水平，而最高表达水平在女性15天内出现。组织表达模式分析表明，在所有测试组织中，在所有测试组织中表达，在雄性的消化道中表达了相对较高的表达水平，但雌性没有显着的组织差异。首次采用RNA干扰策略来诱导更大的抑制 MCFPPS </ I> mRNA，因此下游基因的表达水平和产物浓度的急剧下降。所有这些结果表明， MCFPPS </ i>可以直接涉及或在Cantharidin的生物合成中发挥重要作用。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2017年第5期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zha Shenfang&option=202" target="_blank" rel="nofollow">Zha Shenfang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yin Youping&option=202" target="_blank" rel="nofollow">Yin Youping;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Yu&option=202" target="_blank" rel="nofollow">Wang Yu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Yi&option=202" target="_blank" rel="nofollow">Huang Yi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Yan&option=202" target="_blank" rel="nofollow">Li Yan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Zhongkang&option=202" target="_blank" rel="nofollow">Wang Zhongkang;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Chongqing Engineering Research Center for Fungal InsecticideChongqing UniversityChongqing People's Republic of China;</p> <p>Chongqing Engineering Research Center for Fungal InsecticideChongqing UniversityChongqing People's Republic of China;</p> <p>Chongqing Engineering Research Center for Fungal InsecticideChongqing UniversityChongqing People's Republic of China;</p> <p>Chongqing Engineering Research Center for Fungal InsecticideChongqing UniversityChongqing People's Republic of China;</p> <p>Chongqing Engineering Research Center for Fungal InsecticideChongqing UniversityChongqing People's Republic of China;</p> <p>Chongqing Engineering Research Center for Fungal InsecticideChongqing UniversityChongqing People's Republic of China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cantharidin&option=203" rel="nofollow">cantharidin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=farnesyl pyrophosphate synthase&option=203" rel="nofollow">farnesyl pyrophosphate synthase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mylabris cichorii&option=203" rel="nofollow">Mylabris cichorii;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=RNA interference&option=203" rel="nofollow">RNA interference;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gene function&option=203" rel="nofollow">gene function;</a> </p> <div class="translation"> 机译：Cantharidin;法呢基焦磷酸盐合成酶;mylabris cichorii;RNA干扰;基因功能; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881115.html">Cloning and functional analysis of farnesyl pyrophosphate synthase (FPPS) gene from <i xmlns="http://www.wiley.com/namespaces/wiley">Mylabris cichoriiMylabris cichorii</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zha Shenfang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zha Shenfang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yin Youping&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yin 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=彭世清&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,彭世清</a> <span> <a href="/journal-cn-6832/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 热带农业工程 </a> </span> <span> . 2010</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315084283.html">眼斑芫菁法呢基焦磷酸合酶FPPS基因的克隆及功能研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=查神芳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 查神芳</a> <span> . 2015</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201752202.html">玉麦法呢基焦磷酸合酶基因YFPS及其分离克隆、定点突变及酶功能的检测方法</a> <b>[P]</b> . <span> 中国专利： CN102994527B </span> <span> . 2015.08.26</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120101689687.html">玉麦法呢基焦磷酸合酶基因YFPS及其分离克隆、定点突变及酶功能的检测方法</a> <b>[P]</b> . <span> 中国专利： CN102994527A </span> <span> . 2013-03-27</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130447536968.html">PLANT VECTOR HAVING SUNFLOWER FARNESYL PYROPHOSPHATE SYNTHASE GENE AND PROCESS FOR PRODUCING THE SAME</a> <b>[P]</b> . <span> 外国专利：  JP2002306005A </span> <span> . 2002-10-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有葵花型焦磷酸正磷酸酯合成酶基因的植物及其生产方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130449138931.html">PLANT VECTOR HAVING SUNFLOWER FARNESYL PYROPHOSPHATE SYNTHASE GENE AND METHOD FOR PRODUCING THE SAME</a> <b>[P]</b> . <span> 外国专利：  JP2001000192A </span> <span> . 2001-01-09</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有葵花型磷酸正戊酯合成酶基因的植物及其生产方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704021881115','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> 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Cloning and functional analysis of farnesyl pyrophosphate synthase (FPPS) gene from Mylabris cichoriiMylabris cichorii

摘要