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首页> 外文期刊>Cytokine >ELR(+) CXC chemokines in human milk.
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ELR(+) CXC chemokines in human milk.

机译：人乳中的ELR（+）CXC趋化因子。

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摘要

CXC chemokines bearing the glutamic acid-leucine-arginine (ELR) motif are crucial mediators in neutrophil-dependent acute inflammation. Interestingly, however, Interleukin (IL)-8/CXC ligand (CXCL) 8 is expressed in human milk in biologically significant concentrations, and may play a local maturational role in the developing human intestine. In this chemokine subfamily, there are six other known peptides beside IL-8/CXCL8, all sharing similar effects on neutrophil chemotaxis and angiogenesis. In this study, we measured the concentrations of these chemokines in human milk, sought their presence in human mammary tissue by immunohistochemistry, and confirmed chemokine expression in cultured human mammary epithelial cells (HMECs). Each of the seven ELR(+) CXC chemokines was measurable in milk, and except for NAP-2/CXCL7, these concentrations were higher than serum. The concentrations were higher in colostrum (except for GRO-beta/CXCL2 and NAP-2/CXCL7), and correlated negatively with time elapsed postpartum. IL-8/CXCL8, GRO-gamma/CXCL3, and ENA-78/CXCL5 concentrations were higher in preterm milk. There was intense immunoreactivity in mammary epithelial cells for all ELR(+) CXC chemokines, and the intensity of staining was higher in breast tissue with lactational changes. The supernatants from confluent HMEC cultures also contained measurable concentrations of all the seven ELR(+) CXC chemokines. These results confirm that all ELR(+) CXC chemokines are actively secreted by the mammary epithelial cells into human milk. Further studies are needed to determine if these chemokines share with IL-8/CXCL8 the protective effects on intestinal epithelial cells.
机译：带有谷氨酸-亮氨酸-精氨酸（ELR）基序的CXC趋化因子是中性粒细胞依赖性急性炎症的关键介质。然而，有趣的是，白介素（IL）-8 / CXC配体（CXCL）8在人乳中以生物学上显着的浓度表达，并且可能在发育中的人肠中发挥局部成熟作用。在这个趋化因子亚家族中，除IL-8 / CXCL8外，还有其他六种已知的肽，它们均对嗜中性白细胞趋化性和血管生成具有相似的作用。在这项研究中，我们测量了人乳中这些趋化因子的浓度，通过免疫组织化学法寻找它们在人乳腺组织中的存在，并在培养的人乳腺上皮细胞（HMECs）中证实了趋化因子的表达。牛奶中可测量7种ELR（+）CXC趋化因子中的每一种，除NAP-2 / CXCL7外，这些浓度均高于血清。初乳中的浓度较高（GRO-β/ CXCL2和NAP-2 / CXCL7除外），并且与产后时间呈负相关。早产牛奶中的IL-8 / CXCL8，GRO-γ/ CXCL3和ENA-78 / CXCL5浓度较高。乳腺上皮细胞对所有ELR（+）CXC趋化因子均具有强烈的免疫反应性，并且随着泌乳期的变化，乳腺组织的染色强度更高。来自汇合的HMEC培养物的上清液还包含所有七个ELR（+）CXC趋化因子的可测量浓度。这些结果证实，所有的ELR（+）CXC趋化因子均由乳腺上皮细胞主动分泌到人乳中。需要进一步的研究以确定这些趋化因子是否与IL-8 / CXCL8共享对肠上皮细胞的保护作用。

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