Systematic interpolation method predicts protein chromatographic elution with salt gradients, pH gradients and combined salt/pH gradients

Creasy Arch; Barker Gregory; Carta Giorgio

首页> 外文期刊>Biotechnology Journal: Healthcare,Nutrition,Technology >Systematic interpolation method predicts protein chromatographic elution with salt gradients, pH gradients and combined salt/pH gradients

【24h】

Systematic interpolation method predicts protein chromatographic elution with salt gradients, pH gradients and combined salt/pH gradients

机译：系统的内插方法通过盐梯度，pH梯度和组合盐/ pH梯度预测蛋白质色谱洗脱

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A methodology is presented to predict protein elution behavior from an ion exchange column using both individual or combined pH and salt gradients based on high-throughput batch isotherm data. The buffer compositions are first optimized to generate linear pH gradients from pH 5.5 to 7 with defined concentrations of sodium chloride. Next, high-throughput batch isotherm data are collected for a monoclonal antibody on the cation exchange resin POROS XS over a range of protein concentrations, salt concentrations, and solution pH. Finally, a previously developed empirical interpolation (EI) method is extended to describe protein binding as a function of the protein and salt concentration and solution pH without using an explicit isotherm model. The interpolated isotherm data are then used with a lumped kinetic model to predict the protein elution behavior. Experimental results obtained for laboratory scale columns show excellent agreement with the predicted elution curves for both individual or combined pH and salt gradients at protein loads up to 45 mg/mL of column. Numerical studies show that the model predictions are robust as long as the isotherm data cover the range of mobile phase compositions where the protein actually elutes from the column.

机译：提出一种方法来预测来自离子交换柱的蛋白质洗脱行为，使用基于高吞吐量批量等温数据的单独或组合的pH和盐梯度来预测离子交换柱。首先优化缓冲剂组合物以产生从pH 5.5至7的线性pH梯度，具有定义的氯化钠浓度。接下来，在一系列蛋白质浓度，盐浓度和溶液pH上，在阳离子交换树脂孔X上的单克隆抗体收集高通量批次等温数据。最后，以前开发的经验插值（EI）方法延伸以描述蛋白质结合作为蛋白质和盐浓度和溶液pH的函数而不使用显式等温模型。然后将内插等温数据与集体的动力学模型一起使用以预测蛋白质洗脱行为。实验室规模柱获得的实验结果表明，在蛋白质载荷上的单个或合并的pH和盐梯度的预测洗脱曲线均显示出优于45mg / ml的柱的最佳协议。数值研究表明，只要等温数据覆盖了蛋白质实际从柱子拍摄的流动相组合物的范围，模型预测是稳健的。

著录项

来源
《Biotechnology Journal: Healthcare,Nutrition,Technology》 |2017年第3期|共11页
作者
Creasy Arch; Barker Gregory; Carta Giorgio;
展开▼
作者单位

Univ Virginia Dept Chem Engn 102 Engn Way Charlottesville VA 22904 USA;

Bristol Myers Squibb Biol Proc Dev Hopewell NJ USA;

Univ Virginia Dept Chem Engn 102 Engn Way Charlottesville VA 22904 USA;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;
关键词
Ion exchange chromatography; Linear pH gradients; Linear salt gradients; Predictive modeling; Proteins;

机译：离子交换色谱;线性pH梯度;线性盐梯度;预测建模;蛋白质;

相似文献

外文文献
中文文献
专利

1. Systematic interpolation method predicts protein chromatographic elution with salt gradients, pH gradients and combined salt/pH gradients [J] . Creasy Arch, Barker Gregory, Carta Giorgio Biotechnology Journal: Healthcare,Nutrition,Technology . 2017,第3期

机译：系统的内插方法通过盐梯度，pH梯度和组合盐/ pH梯度预测蛋白质色谱洗脱
2. Systematic Interpolation Method Predicts Antibody Monomer-Dimer Separation by Gradient Elution Chromatography at High Protein Loads [J] . Creasy Arch, Reck Jason, Pabst Timothy, Biotechnology Journal: Healthcare,Nutrition,Technology . 2019,第3期

机译：系统内插方法通过高蛋白质负荷通过梯度洗脱色谱法预测抗体单体二聚体分离
3. Optimization of elution salt concentration in stepwise elution of protein chromatography using linear gradient elution data - Reducing residual protein A by cation-exchange chromatography in monoclonal antibody purification [J] . Ishihara T, Kadoya T, Endo N, Journal of chromatography, A: Including electrophoresis and other separation methods . 2006,第1期

机译：使用线性梯度洗脱数据优化蛋白质色谱逐步洗脱中的洗脱盐浓度-通过阳离子交换色谱减少单克隆抗体纯化中的残留蛋白A
4. Temperature Gradient Induced Caking of Pharmaceutical Grade Salt [C] . A.H.J.Paterson, K.J.Anthony, J.E.Bronlund 40th Annual Loss Prevention Symposium . 2006

机译：温度梯度诱导的药用盐结块
5. Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes. [D] . Dutta, Partha. 2006

机译：使用PCR扩增的16S rRNA基因的变性梯度凝胶电泳，沿着盐梯度进行土壤群落分析。
6. Systematic Interpolation Method Predicts Protein Chromatographic Elution from Batch Isotherm Data without a Detailed Mechanistic Isotherm Model [O] . Arch Creasy, Gregory Barker, Yan Yao, -1

机译：系统内插法可从批量等温线数据预测蛋白质色谱洗脱而无需详细的等温等温线模型
7. P981Lvot area measurement using gated ct data reclassifies aortic stenosis severity as graded by echocardiographyP982Paradoxical low-flow low-gradient aortic stenosis: an intermediate state between moderate and severe aortic stenosis?P983Can rheumatic significant mitral stenosis be a cause of paradoxical low gradient, low flow, in patients with severe aortic stenosis? an echocardiographic and outcome studyP984Clinical and hemodynamic comparison of isolated versus combined aortic and mitral stenosisP985Echocardiographic end-diastolic velocity in the proximal descending aorta should be interpreted with caution when the ascending aorta is dilated: insights from cardiovascular magnetic resonanceP987Prevalence of atrial mitral regurgitation in patients with severe mitral regurgitationP988Role of 2D/3D echocardiography in the risk stratification of endocardial lead-related tricuspid regurgitation: a single-centre study among?241 patientsP989When TEE is needed in patients with staphylococcus aureus bacteremia for the assessment of risk profile of infective endocarditis?P990Appropriateness criteria to echocardiograms for suspected infective endocarditis: experience of a tertiary referral centerP991Independent predictors of outcome in infective endocarditisP992The role of transesophageal cardiography in clinical course and prognosis of complicated infective endocarditis in critically ill patients: our 15 years experienceP993Left bundle branch block atypical pattern as a prognostic determinant in patients taken to TAVIP994Efficacy of long-term ivabradine therapy in severe systolic chronic heart failure patients with and without type 2 diabetes mellitusP995Relations between left ventricular reverse remodeling and serum markers of extracellular matrix fibrosis in dilated cardiomyopathyP996The healthy left ventricle accommodates an increasing vortex formation time for volume transfer in diastolic filling :Implications for heart failureP997Evolutionary changes of pulmonary artery pressure after left ventricular assist device implantP998Functional correlates and prognostic value of coronary flow velocity reserve by vasodilator stress echocardiography in hypertrophic cardiomyopathyP999Quantification of myocardial performance in patients with non-obstructive versus latent-obstructive hypertrophic cardiomyopathyP1000Lifelong arrhythmic risk stratification in arrhythmogenic right ventricular cardiomyopathy: distribution of events and impact of periodical reassessmentP1001Impact of fibrosis visualized by CMR in vectorcardiogram recordings of patients with suspected arrhythmogenic cardiomyopathyP1002Determinants of the beneficial effect of aldosterone antagonism on exercise capacity in heart failure with reduced ejection fractionP1003Myocardial strain values in patients with acute myocarditis and preserved ejection fraction. A magnetic resonance feature tracking studyP1004Detection of subclinical left ventricular dysfunction by speckle tracking echocardiography in patients with myocarditis without prominent wall motion abnormalitiesP1005Aborted sudden cardiac death patients aged <50 years show only mild alterations on cardiac magnetic resonance imagingP1006Relationships between subepicardial and subendocardial longitudinal strain with late gadolinium enhancement in uncomplicated hypertensive patients [O] . L. Moderato, C. Di Nora, A. Soufiani, 2016

机译：P981LVOT区域测量使用门控CT数据重新分类主动脉狭窄的严重程度，以超声心动图7982分类为分类，如二醇的低流量低梯度主动脉狭窄：中度和严重主动脉狭窄之间的中间状态？P983CAN风湿显着二尖瓣狭窄是矛盾的低梯度，低流量的原因在严重主动脉狭窄的患者中？超声心动图和结合分离的主动脉和二尖瓣术和二尖瓣狭窄的血液动力学比较的超声心动图和血液动力学比较在近期下降主动脉中应当谨慎地解释升高的主动脉：从心血管磁共振的洞察中的心血管磁共振PREValence在严重的患者中的洞察中解释二尖瓣regurgitationP988 rool 2D / 3D超声心动图在内膜内铅相关三尖瓣反流的风险分层：241例患者中的单一学习，在葡萄球菌的患者中需要TEE，用于评估感染性心内炎的风险概况？P990姑息度标准怀疑感染心内膜炎的超声心动图：第三节推荐中心的经验，感染endocardisp992在感染性Endocardisap999中的临床过程中的作用和复杂感染的预后的作用生病患者的心内膜炎：我们的15年经验训练束分支块的非典型模式作为患者的预后决定因素，以TaviP994患者在严重的收缩期慢性心力衰竭患者中患者，无型糖尿病患者左心室反向重塑和血清基质纤维化的血清标志物在扩张心肌脑肿瘤中，健康的左心室容纳舒张填充中体积转移的增加的涡旋形成时间：对左心室辅助装置Implantp998函数相关和冠状动脉速率储备的肺动脉压的肺动脉压的影响。血管扩张器应力超声心动图在肥厚性心肌病型499中，非阻塞性患者心肌表现与潜在阻塞性肥厚性心肌病的患者患者患者患者患者血小板治疗1000Lifelong心律失常风险Strati心律病学右心室心肌病的发动机：CMR患者血管瘤术治疗患者血管动脉瘤患者血管诊断患者血管心目记录中CMR的纤维化术治疗的事件和影响患有急性心肌炎和保存的喷射分数。磁共振特征跟踪STOPYP1004DETTECTECTECTET通过突出壁运动患者的斑点左心室功能障碍的亚临床左心室功能障碍，没有突出的壁运动异常，P1005aborted突发的心脏死亡患者<50岁的突然性心脏死亡患者只显示心脏磁共振术中的轻度改变，钆和肾外腺纵向应变之间的心脏磁共振成像P1006相关性简单的高血压患者增强
8. Radioactive Waste Isolation in Salt: Geochemistry of Brine in Rock Salt in Temperature Gradients and gamma-Radiation Fields - a Selective Annotated Bibliography [R] . Hull, A. B., Williams, L. B. 1985

机译：盐中放射性废物的分离：温度梯度和γ辐射场中盐水中盐水的地球化学 - 选择性注释书目

Systematic interpolation method predicts protein chromatographic elution with salt gradients, pH gradients and combined salt/pH gradients

摘要

著录项

相似文献

相关主题

期刊订阅