• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Systematic Interpolation Method Predicts Protein Chromatographic Elution from Batch Isotherm Data without a Detailed Mechanistic Isotherm Model
【2h】

Systematic Interpolation Method Predicts Protein Chromatographic Elution from Batch Isotherm Data without a Detailed Mechanistic Isotherm Model

机译：系统内插法可从批量等温线数据预测蛋白质色谱洗脱而无需详细的等温等温线模型

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A method is developed to predict protein chromatographic behavior from batch isotherm using a a systematic empirical interpolation (EI) scheme and without relying on a mechanistic description of the dependence of protein binding on protein and salt concentration. Coupled with a lumped kinetic model with rate parameters determined from HETP measurements for non-binding conditions, the EI scheme is used to numerically predict the column behavior. For two experimental cation exchange systems considered in this work, lysozyme on SP-Sepharose-FF and a monoclonal antibody on POROS XS, predictions based on the EI scheme are in excellent agreement with experimental elution profiles under highly overloaded conditions without using any adjustable parameters. A qualitative study of the sensitivity of predicting protein elution profiles to the precision, granularity, and extent of the batch adsorption data is conducted. Based on the results for a hypothetical system, whose properties are comparable to those found in practice for protein cation exchange chromatography the results of this show that the interpolation scheme is relatively insensitive, requiring only that the ranges of protein and salt concentrations in the experimental dataset overlap those under which the protein actually elutes from the column and along with a 10% measurement precision.
机译：开发了一种使用系统的经验插值（EI）方案从批次等温线预测蛋白质色谱行为的方法，而无需依赖于蛋白质结合对蛋白质和盐浓度的依赖性的机械描述。结合集总动力学模型和速率参数，该速率模型由针对非结合条件的HETP测量确定，EI方案用于数字预测色谱柱行为。对于这项工作中考虑的两个实验性阳离子交换系统，SP-Sepharose-FF上的溶菌酶和POROS XS上的单克隆抗体，基于EI方案的预测与高度过载条件下的实验洗脱曲线非常吻合，无需使用任何可调参数。进行了定性研究，预测蛋白质洗脱曲线对批次吸附数据的精度，粒度和范围的敏感性。根据假设系统的结果，该系统的性质与蛋白质阳离子交换色谱法的实践结果相当，此结果表明插值方案相对不敏感，只需要实验数据集中的蛋白质和盐浓度范围即可与蛋白质实际从色谱柱上洗脱下来的那些重叠，并具有10％的测量精度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号