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首页> 外文期刊>Cytokine >Increased monocyte MCP-1 production in acute alcoholic hepatitis.
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Increased monocyte MCP-1 production in acute alcoholic hepatitis.

机译：在急性酒精性肝炎中单核细胞MCP-1产量增加。

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Monocyte chemoattractant protein-1 (MCP-1) is a potent mononuclear cell-specific chemotactic protein. MCP-1 is a candidate chemoattractant for activation and hepatic infiltration of mononuclear cells in alcoholic hepatitis (AH). Blood was collected from 15 patients with AH (mean bilirubin 17.6+/-3.5 mg/dl; normal 0. 2-1.0 mg/dl) on admission and at time points for up to 6 months. Peripheral blood monocytes were isolated and MCP-1 production assessed by measuring MCP-1 concentrations in monocyte culture supernatants after overnight (20 h) incubation. Monocytes from normal subjects did not product detectable MCP-1 unless stimulated with endotoxin (LPS;5 microg/ml). The mean level of constitutive MCP-1 from AH patient monocytes was 4694+/-2432 pg/ml 20 h on admission. The mean MCP-1 level for LPS-treated monocytes was 4903+/-1540 pg/ml 20 h for normal subjects and was significantly elevated in AH patients to 11589+/-3266 pg/ml/20 h. AH patient monocyte MCP-1 production was decreased in vitro when monocytes were treated with N-acetylcysteine (5 mM) and also decreased over the 6-month study as the patients improved clinically. MCP-1 plasma levels were below the detection limits of the assay used in both AH patients and normal subjects. Thus, monocytes from AH patients not only constitutively product MCP-1, but also produce higher levels of MCP-1 with endotoxin stimulation. Further studies are needed to clarify the role of MCP-1 in the activation and hepatic infiltration of mononuclear cells in alcoholic liver disease. Copyright 1999 Academic Press.
机译：单核细胞趋化蛋白-1（MCP-1）是一种有效的单核细胞特异性趋化蛋白。 MCP-1是酒精性肝炎（AH）中单个核细胞激活和肝浸润的候选化学吸引剂。在入院时以及在长达6个月的时间点，从15例AH患者（平均胆红素17.6 +/- 3.5 mg / dl;正常值0. 2-1.0 mg / dl）中收集血液。孵育过夜（20小时）后，分离外周血单核细胞，并通过测量单核细胞培养上清液中的MCP-1浓度来评估MCP-1的产生。除非受内毒素（LPS; 5微克/毫升）刺激，否则来自正常受试者的单核细胞不会产生可检测的MCP-1。入院20 h，来自AH患者单核细胞的组成型MCP-1的平均水平为4694 +/- 2432 pg / ml。对于正常受试者，LPS处理的单核细胞的平均MCP-1水平在20 h时为4903 +/- 1540 pg / ml，在AH患者中显着升高至11589 +/- 3266 pg / ml / 20 h。当用N-乙酰半胱氨酸（5 mM）处理单核细胞时，AH患者单核细胞MCP-1的产量在体外下降，并且随着患者临床状况的改善，在6个月的研究中也下降。 MCP-1血浆水平低于AH患者和正常受试者的检测限。因此，来自AH患者的单核细胞不仅组成性地产生MCP-1，而且通过内毒素刺激产生更高水平的MCP-1。需要进一步的研究来阐明MCP-1在酒精性肝病中单核细胞的活化和肝浸润中的作用。版权所有1999，学术出版社。

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