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首页> 外文期刊>Cytokine >Regulated expression of alternate transcripts from the mouse oncostatin M gene: implications for interleukin-6 family cytokines.
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Regulated expression of alternate transcripts from the mouse oncostatin M gene: implications for interleukin-6 family cytokines.

机译：小鼠抑癌素M基因的替代转录产物的调控表达：对白介素6家族细胞因子的影响。

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摘要

Oncostatin M (OSM) is a member of the IL-6 family of polyfunctional cytokines. The characterized murine OSM transcript consists of three exons and encodes a secreted protein. Investigations of mOSM expression using the ribonuclease protection assay demonstrated novel sites of expression in undifferentiated but not differentiated pluripotent cells, and revealed the existence of alternatively spliced mOSM transcripts. cDNAs representing a novel mOSM transcript (mOSM 13) containing exon 1 spliced directly to exon 3 were isolated from bone marrow using Rapid Amplification of cDNA Ends (RACE) PCR and RT-PCR approaches. Expression of the mOSM 13 transcript was regulated in a tissue-specific manner and independently of mOSM transcript production, suggesting that its production is biologically significant. Splicing of exon 1 directly to exon 3 disrupts the OSM open reading frame of mOSM 13. Initiation of translation at sites within exon 3 of mOSM 13 would yield N-terminally truncated OSM proteins that are localized within the cell. The omission of exon 2 by alternate splicing and the production of intracellular proteins with alternate biological activities are conserved among several IL-6 family cytokines and are one manifestation of a more general phenomenon; the production of alternate cytokine transcripts encoding intracellular and extracellular proteins. Copyright 2000 Academic Press.
机译：癌抑素M（OSM）是多功能细胞因子IL-6家族的成员。表征的鼠OSM转录本由三个外显子组成，并编码一种分泌的蛋白质。使用核糖核酸酶保护试验对mOSM表达的研究表明，在未分化但未分化的多能细胞中有新的表达位点，并揭示了存在剪接的mOSM转录本。使用cDNA末端快速扩增（RACE）PCR和RT-PCR方法，从骨髓中分离出代表直接包含外显子3的包含外显子1的新颖mOSM转录本（mOSM 13）的cDNA。以组织特异性方式调节mOSM 13转录物的表达，并独立于mOSM转录物的产生，表明其产生具有生物学意义。将外显子1直接剪接至外显子3会破坏mOSM 13的OSM开放阅读框。在mOSM 13的外显子3内的位点启动翻译会产生位于细胞内的N末端截短的OSM蛋白。在几种IL-6家族细胞因子中，通过交替剪接省略外显子2以及产生具有交替生物活性的胞内蛋白是保守的，这是更普遍现象的一种表现。编码细胞内和细胞外蛋白的其他细胞因子转录本的产生。版权所有2000学术出版社。

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