Strong alkaline condition is preferable for producing 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa KNU-2B

Moon Ji-Sun; Lee Yeon-Jung; Kim In-Hwan; Kim Hak-Ryul

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Strong alkaline condition is preferable for producing 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa KNU-2B

机译：优选强碱性条件优选通过假单胞菌铜绿假单胞菌KNU-2B制备来自棕榈酸的7,10-二羟基-8（e )--己二烯酸

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Microbial bioconversion of a given substrate is considered an efficient and eco-friendly tool for value-added industrial compound generation from natural products. Among natural products, unsaturated fatty acids have been used as substrates to produce various functional hydroxy fatty acids. In this study, we report the production of 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid by a new strain, Pseudomonas aeruginosa KNU-2B. KNU-2B efficiently produced DHD from palmitoleic acid and required a strong alkaline condition for maximum DHD production. The maximum DHD amount produced under pH 10.0 and 48-h incubation at 27 degrees C and 150 rpm was 219.5 mg/100 mL culture. Other important nutritional factors were also investigated to obtain optimum DHD production.

机译：给定基质的微生物生物转化被认为是一种高效且生态友好的工具，用于自然产物的增值工业化合物。在天然产物中，不饱和脂肪酸已被用作基材以产生各种官能羟基脂肪酸。在这项研究中，我们通过新的菌株，Pseudomonas铜绿假单胞菌KNU-2b报告了从棕榈酸的7,10-二羟基-8（e )--新苯甲酸（DHD）的产生。 KNU-2B有效地从棕榈原酸中产生DHD，需要强烈的碱性条件，用于最大DHD生产。在27℃和150rpm下在pH10.0和48-H孵育下产生的最大DHD量为219.5mg / 100mL培养物。还研究了其他重要的营养因素，以获得最佳的DHD生产。

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来源
《Biotechnology Letters》 |2020年第9期|共12页
作者
Moon Ji-Sun; Lee Yeon-Jung; Kim In-Hwan; Kim Hak-Ryul;
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作者单位

Kyungpook Natl Univ Sch Food Sci &

Biotechnol Daegu 41566 South Korea;

Kyungpook Natl Univ Sch Food Sci &

Biotechnol Daegu 41566 South Korea;

Korea Univ Grad Sch Dept Publ Hlth Sci Seoul South Korea;

Kyungpook Natl Univ Sch Food Sci &

Biotechnol Daegu 41566 South Korea;

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原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
Palmitoleic acid; Dihydroxy fatty acid; Alkaline condition; Bioconversion; Pseudomonas aeruginosa KNU-2B;

机译：棕榈酸;二羟基脂肪酸;碱性条件;生物转化;假单胞菌铜绿假单胞菌KNU-2B;

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1. Strong alkaline condition is preferable for producing 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa KNU-2B [J] . Moon Ji-Sun, Lee Yeon-Jung, Kim In-Hwan, Biotechnology Letters . 2020,第9期

机译：优选强碱性条件优选通过假单胞菌铜绿假单胞菌KNU-2B制备来自棕榈酸的7,10-二羟基-8（e )--己二烯酸
2. Production and identification of a novel compound, 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3 [J] . Bae JH, Kim DS, Suh MJ, Applied Microbiology and Biotechnology . 2007,第2期

机译：铜绿假单胞菌PR3从棕榈油酸生产和鉴定新型化合物7,10-二羟基-8（E）-十六碳烯酸
3. Production and identification of a novel compound, 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3 [J] . Jae-Han Bae, Deuk-Soo Kim, Min-Jung Suh, Applied Microbiology and Biotechnology . 2007,第2期

机译：铜绿假单胞菌PR3从棕榈油酸生产和鉴定新型化合物7,10-二羟基-8（E）-十六碳烯酸
4. Modeling Framework for investigating the Influence of Amino Acids on the Planktonic-Biofilm Transition of Pseudomonas aeruginosa [C] . Zhaobin Xu, Xin Fang, Thomas K. Wood, IFAC World Congress . 2014

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5. Fatty acid oxidation in Pseudomonas and expression and partial DNA sequence of acyl-CoA synthetase in Pseudomonas aeruginosa PAK. [D] . Leverone, Marianne Rodgers. 1996

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6. The γ-aminobutyric acid-producing ability under low pH conditions of lactic acid bacteria isolated from traditional fermented foods of Ishikawa Prefecture Japan with a strong ability to produce ACE-inhibitory peptides [O] . Florin Barla, Takashi Koyanagi, Naoko Tokuda, 2016

机译：从日本石川县传统发酵食品中分离出的乳酸菌在低pH条件下的γ-氨基丁酸生产能力具有很强的产生ACE抑制肽的能力
7. A simple test for the detection of KPC and metallo-β-lactamase carbapenemase-producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin [O] . Pasteran F., Veliz O., Faccone D., 2011

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8. D-Amino Acids Enhance the Activity of Antimicrobials against Biofilms of Clinical Wound Isolates of Staphylococcus aureus and Pseudomonas aeruginosa. [R] . Sanchez, J., Akers, K. S., Romano, D. R., 2014

机译：D-氨基酸增强抗微生物剂对金黄色葡萄球菌和铜绿假单胞菌临床伤口分离物生物膜的活性。

Strong alkaline condition is preferable for producing 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa KNU-2B

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