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首页> 外文期刊>Cytokine >Effects of TGF-beta1 on interleukin profile of human dental pulp and odontoblasts.
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Effects of TGF-beta1 on interleukin profile of human dental pulp and odontoblasts.

机译：TGF-beta1对人牙髓和成牙本质细胞白介素谱的影响。

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Transforming growth factor-beta 1 (TGF-beta1) is the most extensively studied growth factor in dentin-pulp complex, with pleiotropic effects on pulp response and healing. Our main objective was to analyze the expression profile of pulp tissue and odontoblasts, and the effects of TGF-beta1 on these profiles in cultured human pulp and odontoblasts with a specific interest in the anti- and pro-inflammatory cytokines. For that purpose, pulps and odontoblasts were cultured for different time periods, and microarray was performed to both cultured and native samples. Of cytokines, various interleukins (IL) were confirmed by RT-PCR, and in +/- TGF-beta1 treated pulps also by antibody array. Pro-inflammatory IL-7, -12alpha and -16 mRNAs were detected in native pulp. The expression levels of pro-inflammatory IL-1alpha, -1beta, -6 and -8 were clearly induced after TGF-beta1 treatment, while no anti-inflammatory cytokines were induced. Of all pulpal interleukins analyzed IL-6 and -8 were present at the highest levels in conditioned pulp tissue media. In native odontoblasts pro-inflammatory IL-6 and -7 mRNAs were detected, and in cultured odontoblasts pro-inflammatory IL-8 mRNA showed over 20-fold transient induction after TGF-beta1 treatment. Our results demonstrate that TGF-beta1 is a potent regulator of pro-inflammatory responses and defensive reactions in dentin-pulp complex.
机译：转化生长因子-beta 1（TGF-beta1）是牙本质-纸浆复合物中研究最广泛的生长因子，对纸浆反应和愈合具有多效作用。我们的主要目的是分析牙髓组织和成牙本质细胞的表达谱，以及TGF-β1对培养的人类牙髓和成牙本质细胞中这些谱图的影响，其中抗炎和促炎性细胞因子具有特殊的意义。为此，将纸浆和成牙本质细胞培养不同的时间，并对培养的样品和天然样品进行微阵列分析。在细胞因子中，通过RT-PCR证实了各种白介素（IL），在+/- TGF-beta1处理的果肉中也通过抗体阵列证实了。在天然牙髓中检测到促炎性IL-7，-12alpha和-16 mRNA。 TGF-β1处理后，明显诱导了促炎性IL-1alpha，-1beta，-6和-8的表达水平，而未诱导出抗炎性细胞因子。在所有经分析的牙髓白介素中，IL-6和-8在经调节的牙髓组织培养基中含量最高。在天然成牙本质细胞中检测到促炎性IL-6和-7 mRNAs，在培养的成牙本质细胞中促炎性IL-8 mRNA在TGF-β1处理后显示出超过20倍的瞬时诱导。我们的结果表明，TGF-beta1是牙本质浆复合物中促炎反应和防御反应的有效调节剂。

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