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首页> 外文期刊>Cytokine >Monocyte migration inhibitory factor synthesis and gene expression in particle-activated macrophages.
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Monocyte migration inhibitory factor synthesis and gene expression in particle-activated macrophages.

机译:单核细胞迁移抑制因子合成和颗粒激活巨噬细胞中的基因表达。

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摘要

This study analysed MIF mRNA and protein expression in human macrophages exposed in vitro to polymethylmethacryate and titanium alloy particles. MIF levels released from macrophages without exposure to particles were in the range of 2-4 ng/ml. Exposure of macrophages to particles as demonstrated increased MIF release at 0. 075%-0.225% v/v particle concentration, which was maximal at 12-24 h. MIF mRNA signal levels in cells with and without particles at a concentration of 0.075% showed no significant differences in a time course experiment. The profile of MIF release in response to increasing particle concentration coincided with increased release of lactate dehydrogenase. The viability of the cells was unchanged by the addition of particles as determined by 3H-thymidine uptake. These data suggest that MIF expression may represent an independent macrophage response to locally high particle concentrations. Copyright 2000 Academic Press.
机译:这项研究分析了体外暴露于聚甲基丙烯酸甲酯和钛合金颗粒的人巨噬细胞中的MIF mRNA和蛋白表达。从巨噬细胞释放而不暴露于颗粒的MIF水平在2-4ng / ml的范围内。如图所示,巨噬细胞暴露于颗粒会增加MIF在0. 075%-0.225%v / v颗粒浓度下的释放,这在12-24 h时最大。在有和没有颗粒的细胞中,浓度为0.075%的细胞中的MIF mRNA信号水平在时程实验中没有显示出显着差异。响应于增加的颗粒浓度的MIF释放曲线与乳酸脱氢酶释放增加相吻合。如通过3H-胸腺嘧啶核苷摄取所确定的,通过添加颗粒,细胞的生存力没有改变。这些数据表明,MIF表达可能代表对局部高颗粒浓度的独立巨噬细胞反应。版权所有2000学术出版社。

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