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首页> 外文期刊>Cytokine >HO-1 and VEGF gene expressions are time dependant during exposure to welding fumes.
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HO-1 and VEGF gene expressions are time dependant during exposure to welding fumes.

机译：在暴露于焊接烟雾中，HO-1和VEGF基因的表达与时间有关。

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Hemeoxygenase-1 (HO-1) is a defensive enzyme against oxidative stress. Vascular epithelial growth factor (VEGF) is a potent cytokine which promotes angiogenesis. We used induced sputum (IS) technology to study HO-1 and VEGF expressions in neutrophilic inflammation in asymptomatic welders. Aircraft plant employees were divided into three groups: Welders 1 (n=30) had short-term exposure to aluminum/iron, Welders 2 (n=16) had long-term exposure to cadmium/chromium/ironickel, and controls (n=27 non-exposed individuals). Participants underwent pulmonary function tests (PFTs), IS, differential cell counts, and determination of particle size distribution in IS samples. HO-1 and VEGF gene expressions were analyzed by real-time polymerase chain reaction, and protein levels were measured by bilirubin reductase-dependant reaction and ELISA, respectively. All subjects had normal PFTs. Welders 2 had neutrophilic inflammation and higher percentages of particles between 2-5 micron than the other groups. HO-1 inversely correlated with VEGF gene expression: HO-1 was significantly higher and VEGF was significantly lower in the Welders 1 group than in the other groups. There was a correlation between HO-1 expression and protein activity (r=0.33, P=0.05). Particulate matters significantly influenced HO-1 and VEGF gene expressions, caused neutrophilic inflammation and promoted oxidative stress in welders with long-term exposure.
机译：血氧合酶-1（HO-1）是一种抗氧化应激的防御酶。血管上皮生长因子（VEGF）是一种有效的细胞因子，可促进血管生成。我们使用诱导痰（IS）技术研究无症状焊工中性粒细胞炎症中HO-1和VEGF的表达。飞机工厂的员工分为三类：焊工1（n = 30）短期暴露于铝/铁，焊工2（n = 16）长期暴露于镉/铬/铁/镍，而对照组（ n = 27个未暴露的个体）。参加者进行了肺功能测试（PFTs），IS，差细胞计数以及IS样品粒径分布的测定。通过实时聚合酶链反应分析HO-1和VEGF基因的表达，分别通过胆红素还原酶依赖性反应和ELISA测定蛋白水平。所有受试者的PFT均正常。焊工2具有嗜中性炎症，并且比其他组具有更高的2-5微米之间的颗粒百分比。 HO-1与VEGF基因表达呈负相关：与其他组相比，Welders 1组HO-1显着升高，而VEGF显着降低。 HO-1的表达与蛋白活性之间存在相关性（r = 0.33，P = 0.05）。在长期暴露的焊工中，颗粒物质会显着影响HO-1和VEGF基因的表达，引起嗜中性炎症并促进氧化应激。

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