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首页> 外文期刊>Cytokine >Quantification of Interleukin-6 in cell culture medium using surface plasmon resonance biosensors.
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Quantification of Interleukin-6 in cell culture medium using surface plasmon resonance biosensors.

机译：使用表面等离子体共振生物传感器对细胞培养基中白细胞介素6的定量。

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摘要

Interleukin (IL)-6, a multifunctional cytokine, is widely used as an index for illnesses such as inflammatory and autoimmune disorders, coronary artery disease, neurological disease, and gestational problems. It is thus very important to be able to precisely quantify the level of IL-6 for disease diagnosis and any subsequent therapy. Surface plasmon resonance (SPR) biosensors are sensitive in detecting the interaction between biomolecules by sensing the changes in the refractive index on the sensor chip. This study investigated the SPR technique to determine the IL-6 secretion of human fibroblast MRC5-CVI cells induced by lipopolysaccharide (LPS). To reduce non-specific binding, a mixed self-assembled monolayer of mercaptoundecanoic acid (MUA) and mercaptohexanol (MCH) was attached to the sensor, and then used for IL-6 determination using a sandwich type immunoassay. In addition, two antibody immobilization methods were applied to the sensor surface-direct immobilization and indirect immobilization via protein G affinity. The results demonstrated that the direct immobilization method had a better antibody binding capacity on the sensor surface. The level of cellular IL-6 secretion detected by the SPR biosensor showed a consistent correlation with the commercial kit of IL-6 enzyme-linked immunosorbent assay.
机译：白细胞介素（IL）-6是一种多功能细胞因子，被广泛用作炎症，自身免疫性疾病，冠状动脉疾病，神经系统疾病和妊娠问题等疾病的指标。因此，对于疾病诊断和任何后续治疗而言，能够精确定量IL-6的水平非常重要。表面等离子体激元共振（SPR）生物传感器通过检测传感器芯片上的折射率变化来检测生物分子之间的相互作用非常敏感。这项研究调查了SPR技术，以确定由脂多糖（LPS）诱导的人成纤维细胞MRC5-CVI细胞的IL-6分泌。为了减少非特异性结合，将巯基十一烷酸（MUA）和巯基己醇（MCH）的混合自组装单分子膜连接到传感器，然后使用夹心型免疫测定法进行IL-6测定。另外，将两种抗体固定方法应用于传感器表面直接固定和通过蛋白G亲和力间接固定。结果表明，直接固定方法在传感器表面具有更好的抗体结合能力。 SPR生物传感器检测到的细胞IL-6分泌水平与商业化的IL-6酶联免疫吸附测定试剂盒显示出一致的相关性。

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