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Leptin receptor long-form signalling in a human liver cell line.

机译:人肝细胞系中的瘦素受体长信号传导。

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Expression of the long form of the leptin receptor was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting in the human liver cell line WRL68. Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. WRL68 cells therefore provide the first human in vitro hepatocyte system in which to study leptin receptor-mediated signalling and to elucidate the role of leptin in liver. Copyright 2001 Academic Press.
机译:通过逆转录酶-聚合酶链反应(RT-PCR)和Western印迹法检测人肝细胞系WRL68中瘦素受体的长形式表达。瘦素(50-200 nM)以剂量依赖性方式显着增加STAT细胞质转录因子STAT3和STAT5b的酪氨酸磷酸化,并产生STAT3和STAT5特异性寡核苷酸的凝胶位移。因此,WRL68细胞提供了第一个人类体外肝细胞系统,用于研究瘦素受体介导的信号传导并阐明瘦素在肝脏中的作用。版权所有2001,学术出版社。

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