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首页> 外文期刊>Cytokine >Expression of IL-15 and IL-4 in IFN-gamma-independent control of experimental human Cryptosporidium parvum infection.
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Expression of IL-15 and IL-4 in IFN-gamma-independent control of experimental human Cryptosporidium parvum infection.

机译：IL-15和IL-4在实验性人隐孢子虫感染的IFN-γ独立控制中的表达。

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We have previously demonstrated interferon gamma (IFN-gamma) in intestinal mucosa after experimental human Cryptosporidium parvum infection, but expression was limited to sensitized volunteers. To characterize IFN-gamma-independent mechanisms in control of infection, jejunal biopsies from immunocompetent volunteers experimentally challenged with C. parvum were examined by in situ hybridization for interleukin (IL-)15 and IL-4 mRNA with confirmation by immunohistochemistry. Cytokine expression was correlated with prechallenge anti- C. parvum IgG, symptoms, oocyst shedding, and prior IFN-gamma expression data. IL-15 expression was noted only in those without prior sensitization, who did not express IFN-gamma. By contrast, expression of IL-4 was associated with prior sensitization. IL-15 was only detected in those with symptoms (6/14 symptomatic vs 0/3 asymptomatic, P<0.05). Among 14 volunteers who did not express IFN-gamma, oocyst shedding was lower in those expressing IL-15. Overall, 14/15 volunteers who did not shed oocysts expressed either IFN-gamma or IL-15. There was no correlation between expression of IL-4 and symptoms or oocyst shedding. In conclusion, IL-15 expression was associated with control of oocyst shedding in those not expressing IFN-gamma. These data suggest that IL-15 is involved in IFN-gamma independent mechanisms of control of human cryptosporidiosis, perhaps via activation of the innate immune response. Copyright 2001 Academic Press.
机译：我们先前已经证明了实验性人类隐孢子虫感染后肠道粘膜中的干扰素γ（IFN-γ），但表达仅限于致敏的志愿者。为了表征控制感染的IFN-γ依赖性机制，通过原位杂交白介素（IL-）15和IL-4 mRNA并通过免疫组织化学确认，检查了来自免疫活性志愿者实验性感染小球藻的空肠活检组织。细胞因子的表达与挑战前抗小肠弯曲杆菌IgG，症状，卵囊脱落和先前的IFN-γ表达数据相关。仅在没有先兆致敏的人中表达IL-15表达，这些人不表达IFN-γ。相反，IL-4的表达与先前的致敏有关。仅在有症状（6/14有症状与0/3无症状，P <0.05）的患者中检测到IL-15。在不表达IFN-γ的14位志愿者中，表达IL-15的卵囊脱落率较低。总体而言，未脱落卵囊的14/15名志愿者表达了IFN-γ或IL-15。 IL-4的表达与症状或卵囊脱落没有相关性。总之，IL-15的表达与不表达IFN-γ的卵囊脱落的控制有关。这些数据表明IL-15可能通过激活先天性免疫应答而参与了人隐孢子虫病控制的IFN-γ独立机制。版权所有2001，学术出版社。

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