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A single QTL on chromosome 6DS derived from a winter wheat cultivar 'OW104' confers resistance to Wheat yellow mosaic virus

机译:染色体6ds上的单个QTL来自冬小麦品种'OW104'赋予麦片黄色马赛克病毒的抗性

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Wheat yellow mosaic (WYM) is a soilborne disease caused by Wheat yellow mosaic virus (WYMV). Symptoms include yellow mosaic coloring of leaves, stunting, and growth inhibition. Severe infection may result in yield loss. WYM is one of the most serious diseases affecting wheat production in East Asia. The most effective control is through breeding resistant cultivars. A winter wheat cultivar, 'OW104', shows little to no symptoms in heavily WYMV-infested fields in Hokkaido, Japan. Here we detected Qym4, a QTL accounting for 45%-57% of WYMV resistance, in the vicinity of the markers Xcfd49, Xbarc183, and Xgpw4357 on wheat chromosome arm 6DS. F3 progenies with 'OW104' allele at Qym4 showed significantly higher resistance than those with 'Hokushin' homozygote or heterozygote. We developed 'Hokushin' near-isogenic lines by backcrossing with 'Hokushin' as the recurrent parent and 'OW104' as the resistance donor. All the WYMV-resistant BC5F1/BC4F1 plants carried 'OW104' allele only at Xcfd49. Our results suggest that the introduction of Qym4 confers resistance to WYMV in winter wheat.
机译:小麦黄色马赛克(Wym)是一种由小麦黄色马赛克病毒(WYMV)引起的土壤疾病。症状包括黄色马赛克着色的叶子,眩晕和生长抑制。严重的感染可能导致产量损失。 Wym是影响东亚小麦产量的最严重的疾病之一。最有效的控制是通过养殖抗性品种。冬小麦品种'Ow104',在日本北海道的大量广域Wymv-infested领域没有任何症状。在这里,我们检测到QYM4,QTL占WYMV电阻的45%-57%,在标记XCFD49,Xbarc183和XGPW4357附近,小麦染色体臂6ds。在Qym4的“Ow104”等位基因的F3后代表现出比“Hokushin”纯合子或杂合子的抵抗力显着更高。我们通过回复“Hokushin”作为复发父母和“OW104”作为抵抗供体的回复,我们开发了“Hokushin”近代源线。所有WMV抗性BC5F1 / BC4F1工厂仅在XCFD49携带“OW104”等位基因。我们的研究结果表明,Qym4引入冬小麦赋予WYMV抵抗。

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