首页> 外国专利> METHOD FOR DETECTING ALIEN CHROMOSOME DERIVED FROM CLOSELY-RELATED SPECIES OF WHEAT INTRODUCED INTO WHEAT

METHOD FOR DETECTING ALIEN CHROMOSOME DERIVED FROM CLOSELY-RELATED SPECIES OF WHEAT INTRODUCED INTO WHEAT

机译：检测导入小麦的近缘种的外源染色体的方法

页面导航

摘要
著录项
相似文献

摘要

PPROBLEM TO BE SOLVED: To provide a method for detecting an alien chromosome introduced into wheat, by using a wheat genome as a genetic background. PSOLUTION: This method for detecting the alien chromosome includes an amplification process for amplifying a DNA fragment by using a primer pair designed based on a barley EST sequence as a primer pair and further using a genomic DNA of a test individual of the wheat as a template DNA and an amplified fragment confirmation process for confirming the DNA fragment amplified in the amplification process, wherein many primer pairs which can detect the alien chromosomes derived from 10 closely-related species of the wheat belonging to Triticeae are discovered among primer pairs developed based on the barley EST sequence and used in the method. PCOPYRIGHT: (C)2007,JPO&INPIT

机译：<要解决的问题：提供一种通过使用小麦基因组作为遗传背景来检测引入小麦的外来染色体的方法。

解决方案：这种检测外来染色体的方法包括一个扩增过程，该过程通过使用基于大麦EST序列设计的引物对作为引物对，并进一步使用小麦受试个体的基因组DNA来扩增DNA片段。作为模板DNA和用于确认在扩增过程中扩增的DNA片段的扩增片段确认过程，其中在开发的引物对中发现许多可以检测到属于小麦的10个紧密相关物种的外来染色体的引物对。基于大麦EST序列并在该方法中使用。

版权：（C）2007，日本特许厅＆INPIT 展开▼

著录项

公开/公告号JP2007082429A

专利类型
公开/公告日2007-04-05

原文格式PDF
申请/专利权人 JAPAN SCIENCE & TECHNOLOGY AGENCY;
展开▼

申请/专利号JP20050272667
发明设计人 TAKEDA KAZUYOSHI;SATO KAZUHIRO;
展开▼

申请日2005-09-20
分类号C12Q1/68;C12N15/09;
国家 JP
入库时间 2022-08-21 21:11:23

相似文献

专利
外文文献
中文文献