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首页> 外文期刊>Cytokine >Effect of midazolam on interleukin-6 mRNA expression in human peripheral blood mononuclear cells in the absence of lipopolysaccharide.
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Effect of midazolam on interleukin-6 mRNA expression in human peripheral blood mononuclear cells in the absence of lipopolysaccharide.

机译：在没有脂多糖的情况下，咪达唑仑对人外周血单个核细胞中白细胞介素6 mRNA表达的影响。

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Midazolam, a benzodiazepine, has an hypnotic effect via benzodiazepine receptors and is widely used as an anaesthetic. Recently, it has been suggested that benzodiazepines modulate cytokine responses. The purpose of the present study was to evaluate the effect of midazolam on interleukin-6 (IL-6) response by observing mRNA expression levels in human peripheral blood mononuclear cells (PBMCs) in the absence of lipopolysaccharide (LPS). PBMCs were isolated from healthy volunteers in endotoxin-free 0.9% sodium chloride solution. The cells were incubated for 2 h at 37 degrees C immediately after isolation. IL-6 mRNA expression levels in the cells were quantified using reverse transcription and competitive polymerase chain reaction. It was found that midazolam time-dependently inhibited the IL-6 mRNA expression in PBMCs in the absence of LPS, and significantly inhibited the IL-6 mRNA expression at 1 microg/ml (P<0.05) or 10 microg/ml (P<0.01) in the absence of LPS. However, neither a specific agonist of peripheral-type benzodiazepine receptors, Ro5-4864, nor a specific agonist of central-type benzodiazepine receptors, clonazepam, inhibited IL-6 mRNA expression. These findings indicated a suppression of the IL-6 response in human PBMCs by midazolam in the absence of LPS, and suggests that midazolam has its effect not via benzodiazepine receptors, but by another mechanism. Copyright 2001 Academic Press.
机译：咪达唑仑，一种苯二氮卓类药物，通过苯二氮卓类受体具有催眠作用，被广泛用作麻醉剂。最近，已经提出苯二氮卓类调节细胞因子应答。本研究的目的是通过观察在没有脂多糖（LPS）的情况下人外周血单个核细胞（PBMC）中的mRNA表达水平来评估咪达唑仑对白介素6（IL-6）反应的影响。在无内毒素的0.9％氯化钠溶液中从健康志愿者中分离出PBMC。分离后立即将细胞在37摄氏度下孵育2小时。使用逆转录和竞争性聚合酶链反应定量细胞中IL-6 mRNA的表达水平。发现在不存在LPS的情况下，咪达唑仑可抑制PBMC中IL-6 mRNA的表达，并在1 microg / ml（P <0.05）或10 microg / ml（P < 0.01）在没有LPS的情况下。但是，外周型苯并二氮杂receptor受体的特异性激动剂Ro5-4864或中央型苯并二氮杂receptor受体氯硝西am的特异性激动剂均不能抑制IL-6 mRNA的表达。这些发现表明，在没有LPS的情况下，咪达唑仑抑制了人PBMC中的IL-6反应，并表明咪达唑仑的作用不是通过苯并二氮杂receptor受体，而是通过另一种机制。版权所有2001，学术出版社。

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