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首页> 外文期刊>Cytokine >Dynamic distribution of epidermal growth factor during mouse embryo peri-implantation.
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Dynamic distribution of epidermal growth factor during mouse embryo peri-implantation.

机译：小鼠胚胎围植入过程中表皮生长因子的动态分布。

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摘要

Embryo implantation depends on the synchronized development of the blastocyst and the endometrium. This process is highly controlled by the coordinated action of the steroid hormones: estrogen and progesterone. By autocrine, paracrine or juxtacrine routes, some growth factors or cytokines are involved in this steroidal regulation pathway. Here we report the effects of epidermal growth factor (EGF) on embryo implantation in the mouse, the expression and distribution patterns of EGF protein in the mouse blastocyst, ectoplacental cone (EPC) and peri-implantation uterus on days 1-8 of gestation.By RT-PCR and dot blot, we found that EGF and its receptor (EGFR) are co-expressed in the blastocyst and peri-implantational uteri of pregnant days 2-8 (D2-D8) mice. Injection of EGF antibody into a uterine horn on the third day of pregnancy (D3) significantly reduced the number of mouse embryos that implanted on D8, indicating EGF have a function in the mouse embryo implantation.Further investigation by using indirect immunofluorescence and confocal microscope was made to trace EGF and EGFR protein localization during the mouse embryo implantation. EGF and EGFR are co-localized in the blastocyst, and in the secondary trophoblastic giant cells (SGC) of the EPC. At the pre-implantation stage, the distribution of EGF protein in the mouse uterus changes from epithelium to stroma. On D1 of pregnancy, EGF is mainly distributed in uterine stroma and myometrium. On D2, it is present in the uterine epithelium. On D3, it changes again from the uterine epithelium to the stroma. By D4, EGF is predominantly in the stroma. This dynamic distribution correlates with the proliferation activity of uterine cells at each period. On D6-D8 of embryo implantation, EGF 3 protein accumulates at the uterine mesometrial pole, a region that contributes to the trophoblastic invasiveness and placentation.This temporal and spatial localization of EGF protein in the mouse uterus implicates the cytokine in the regulation of trophoblastic invasiveness and uterine receptiveness.
机译：胚胎植入取决于胚泡和子宫内膜的同步发展。该过程受到类固醇激素（雌激素和孕激素）的协同作用的高度控制。通过自分泌，旁分泌或并列分泌途径，一些生长因子或细胞因子参与该类固醇调节途径。在这里，我们报告了表皮生长因子（EGF）对小鼠胚胎植入的影响，在妊娠1-8天时小鼠胚泡，外胎盘锥体（EPC）和植入周子宫中EGF蛋白的表达和分布模式。通过RT-PCR和斑点印迹，我们发现EGF及其受体（EGFR）在妊娠2-8天（D2-D8）小鼠的胚泡和着床前后子宫中共表达。在妊娠第三天（D3）向子宫角注射EGF抗体可显着减少植入D8的小鼠胚胎的数量，这表明EGF在小鼠胚胎植入中具有功能。使用间接免疫荧光和共聚焦显微镜进行了进一步研究。可以在小鼠胚胎植入过程中追踪EGF和EGFR蛋白的定位。 EGF和EGFR共定位于胚泡和EPC的次级滋养细胞巨细胞（SGC）中。在植入前阶段，小鼠子宫中EGF蛋白的分布从上皮变为间质。在妊娠D1，EGF主要分布在子宫基质和子宫肌层。在D2上，它存在于子宫上皮中。在D3上，它再次从子宫上皮变为间质。通过D4，EGF主要在基质中。该动态分布与每个时期子宫细胞的增殖活性相关。在胚胎植入的D6-D8时，EGF 3蛋白聚集在子宫中膜极，这是滋养细胞浸润和胎盘形成的区域.EGF蛋白在小鼠子宫内的时空定位表明细胞因子参与了滋养细胞浸润的调节和子宫接受性。

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