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Genetic Identification of Dematiaceous Fungi Isolated from Washing Machine in Japan, and Considering of Fungal Removal Methods

机译:日本洗衣机分离的Dematiace真菌的遗传鉴定,考虑真菌去除方法

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摘要

In 2017, 2 household washing machines which were used at a house in Saitama prefecture were tested for fungal flora sampling. The fungi were identified in detail via genetic analysis of the ITS region. The number of fungi which were isolated were 8.7 X 10(6)similar to 6.1 X 10(7) cfu/100cm(2) and dematiaceous fungi such as Ochroconis musae, Exophiala, Cyphellophora, Knufia epidermidis were dominant. As for Knufia epidermidis, this is the first report for identification from a Japanese household. The number of isolated Cladosporium, which is common in the environment, was fewer compared with the above 4 genera. The identified fungi were Cladosporium halatolerans, which is recently being recognized as the main species of Cladosporium. In our research, we have cleaned the 2 washing machines with different bleaches. Residual fungi was detected in washing machine A, which was cleaned with an oxygen bleach. On the other hand, no fungi was detected in washing machine B, which was cleaned with a chlorine bleach.
机译:2017年,测试了2家家庭洗衣机,用于埼玉县的房子,用于真菌植物群采样。通过其区域的遗传分析详细鉴定了真菌。分离的真菌的数量为8.7 x 10(6),类似于6.1 x 10(7)Cfu / 100cm(2)和Devatiace真菌,如Ochroconis Musae,Exosphiala,canphellophora,Knufia表皮是显性的。至于Knufia Epidermidis,这是日本家庭识别的第一个报告。与上述4属相比,在环境中常见的分离型囊孢子孢子孢子的数量较少。所鉴定的真菌是囊孢子孢子酸盐,最近被认为是克拉多孢菌的主要种类。在我们的研究中,我们用不同的漂白剂清洁了2台洗衣机。在洗衣机A中检测到残留的真菌,用氧气漂白清洁。另一方面,在洗衣机B中没有检测到真菌,用氯漂白清洁。

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