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首页> 外文期刊>Cytokine >Extremely low frequency electromagnetic field exposure does not modulate toll-like receptor signaling in human peripheral blood mononuclear cells.
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Extremely low frequency electromagnetic field exposure does not modulate toll-like receptor signaling in human peripheral blood mononuclear cells.

机译：极低频电磁场暴露不会调节人外周血单核细胞中的toll样受体信号。

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摘要

The effects of extremely low frequency electromagnetic fields (ELF-EMF) on human health remain unclear. It has been reported that ELF-EMF may modulate the innate immune response to microorganisms in animal models and mammalian cell-lines. With the recently gained insight in innate immune signaling and the discovery of pattern recognition, we aim to study whether ELF-EMF modulates innate inflammatory signaling pathways. We used human peripheral blood mononuclear cells (PBMCs), isolated from blood from healthy volunteers, which we stimulated with specific TLR2 and TLR4 ligands, and with several microorganisms. The cells were subsequently exposed in B(dc)=3 muT to a highly controlled and standardized ELF-EMF signal (20-5000Hz, B(ac)=5 muT, 30 min) and cytokine production was measured at different time points after stimulation. No significant difference in immune response, as reflected by IL-1beta, IL-6, TNFalpha, IL-8 and IL-10 production, could be detected after stimulation with LPS (TLR4 ligand), Pam3Cys (TLR2 ligand) or a panel of heat killed microorganisms: Mycobacterium tuberculosis, Salmonella typhimurium, Candida albicans, Aspergillus fumigatus and Staphylococcus aureus (multiple TLR ligands). We therefore conclude that under our experimental conditions, ELF-EMF does not modulate the innate immune response of human primary cells after TLR stimulation in vitro.
机译：极低频电磁场（ELF-EMF）对人体健康的影响仍不清楚。据报道，在动物模型和哺乳动物细胞系中，ELF-EMF可以调节对微生物的先天免疫应答。借助最近在先天免疫信号中获得的见识和模式识别的发现，我们旨在研究ELF-EMF是否调节先天性炎症信号通路。我们使用了从健康志愿者的血液中分离出来的人外周血单核细胞（PBMC），我们用特定的TLR2和TLR4配体以及几种微生物对其进行了刺激。随后将细胞在B（dc）= 3 muT下暴露于高度受控和标准化的ELF-EMF信号（20-5000Hz，B（ac）= 5 muT，30分钟），并在刺激后的不同时间点测量细胞因子的产生。用LPS（TLR4配体），Pam3Cys（TLR2配体）或一组LPS刺激后，IL-1beta，IL-6，TNFα，IL-8和IL-10产生的免疫反应没有显着差异。热灭活的微生物：结核分枝杆菌，鼠伤寒沙门氏菌，白色念珠菌，烟曲霉和金黄色葡萄球菌（多个TLR配体）。因此，我们得出结论，在我们的实验条件下，ELF-EMF不会在体外TLR刺激后调节人类原代细胞的先天免疫应答。

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