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Assessment of three strategies for the management of Meloidogyne arenaria on carrot in Mexico using Pochonia chlamydosporia var. mexicana under greenhouse conditions

机译:用Pochonia Chlamydosporia var评估Meloidogyne arenaria在墨西哥胡萝卜术治疗的三种策略。 墨西哥州温室条件下

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Three control strategies for management of Meloidogyne arenaria were evaluated on carrot under greenhouse conditions. The control strategies tested were: i) incorporation of fresh broccoli; ii) carbofuran (Furadan?); and iii) Pochonia chlamydosporia var. mexicana, isolate Pcp21. Each strategy was evaluated separately and in combination (16 treatment combinations), each with three replicates. The experiment was done between February and April 2014 and repeated over the same time period in 2015. Each replicate experimental unit consisted of a 1?kg pot filled with tyndallised soil, to which broccoli, fungus and carbofuran were added according to treatment. Carrot seeds were sown into each pot and inoculated with M.?arenaria eggs according to treatment. Pots were then maintained in the glasshouse for 12 weeks at 25?±?5°C. After this time the fresh root weight, root length, percent reduction in root galling, P.?chlamydosporia var. mexicana colony forming units (CFU)/g of soil, and CFU/g root were all measured. Results showed that isolate Pcp21 of P.?chlamydosporia var. mexicana significantly reduced root galling percentage by 50 and 78% in 2014 and 2015, respectively. Application of the fungus in combination with broccoli or carbofuran also reduced root galling. The number of CFU/ g of soil or root remained the same over the 12 weeks of the experiment. This isolate of P. chlamydosporia significantly reduced damage caused by M. arenaria on carrot and is a promising agent for management of this nematode.
机译:在温室条件下对胡萝卜素苗绿腺癌管理的三种控制策略。测试的控制策略是:i)掺入新鲜的西兰花; II)Carbofuran(Furadan?);和III)Pochonia Chlamydosporia var。墨西哥人,隔离PCP21。每种策略单独评估,并组合(16种治疗组合),每个策略有三个重复。该实验是在2014年2月和4月之间进行的,在2015年的同一时间内重复。每个复制实验单元由一个装满钨的1杆罐组成,根据治疗添加了西兰花,真菌和碳呋喃。胡萝卜种子播种到每个锅中并根据治疗接种用M.? areNaria鸡蛋。然后将罐保持在玻璃池中在25℃±5°C时保持12周。在此时间后,新鲜的根重,根长度,根部粘土减少百分比,p.?Chlamydosporia var。墨西哥菌落形成单位(CFU)/ g土壤和CFU / G根部都是测量的。结果表明,P.?Chlamydosporia VAR的分离物PCP21。 2014年和2015年,墨西哥人将显着减少50%和78%的根本陷入百分比。真菌与西兰花或碳呋喃组合的施用也减少了根gall。在实验的12周内,土壤或根的CFU / g的数量保持不变。这种P.Chlamydosporia的分离物显着降低了CAROT造成的M.Arenaria造成的损害,并且是该线虫管理的有希望的代理。

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