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首页> 外文期刊>Cytokine >Up-regulation of nerve growth factor and interleukin-10 in inflamed and non-inflamed intestinal segments in rats with experimental colitis.
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Up-regulation of nerve growth factor and interleukin-10 in inflamed and non-inflamed intestinal segments in rats with experimental colitis.

机译：实验性结肠炎大鼠发炎和非发炎肠段神经生长因子和白介素10的上调。

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摘要

Inflammatory bowel diseases are characterized by dysregulated immune response to the normal microflora and structural and functional changes of the enteric nervous system which occur in inflamed as well as non-inflamed areas of the bowel. This study describes the changes in the expression of nerve growth factor (NGF) and interleukin-10 (IL-10) in the colon and in various segments of the small intestine in two rat models of experimental colitis induced by iodoacetamide or 2,4,6-trinitrobenzene sulfonic acid (TNBS). Levels of NGF and IL-10 were measured by ELISA in tissue homogenate sampled from duodenum, jejunum, ileum and colon at different time intervals. NGF and IL-10 increased significantly in homogenates of strips isolated from all small intestinal segments, 3-6h after iodoacetamide or TNBS administration and remained elevated until the colonic inflammation subsided. Similar but more pronounced increase occurred in areas of the colon adjacent to the ulcer. Histologic examinations revealed inflammatory changes in the colon; however, examination of sections from the small intestines did not reveal significant differences between controls and rats with colitis. The marked up-regulation of nerve growth factor and interleukin-10 in colitis suggests that they play a role in limiting or resolving inflammation and in preventing it from becoming uncontrolled. It also suggests that experimental colitis may be associated with latent inflammation in the small bowel.
机译：炎症性肠病的特征是对正常菌群的免疫反应失调，以及肠道发炎和非发炎区域中发生的肠道神经系统结构和功能的变化。这项研究描述了两种由碘乙酰胺或2,4诱导的实验性结肠炎大鼠模型在结肠和小肠各个部分中神经生长因子（NGF）和白介素10（IL-10）的表达变化。 6-三硝基苯磺酸（TNBS）。通过ELISA在不同时间间隔从十二指肠，空肠，回肠和结肠采样的组织匀浆中测量NGF和IL-10的水平。在施用碘乙酰胺或TNBS后3至6小时，从所有小肠段分离的条带的匀浆中，NGF和IL-10显着增加，并一直升高直至结肠炎症消退。在溃疡附近的结肠区域发生了类似但更为明显的增加。组织学检查显示结肠中有炎症变化。然而，检查小肠切片并未发现对照组和结肠炎大鼠之间有显着差异。结肠炎中神经生长因子和白介素10的明显上调表明它们在限制或解决炎症以及防止炎症变得不受控制方面发挥作用。这也表明实验性结肠炎可能与小肠潜在的炎症有关。

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