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首页> 外文期刊>Cytokine >Granulocyte-macrophage colony stimulating factor is anabolic and interleukin-1beta is catabolic for rat articular chondrocytes.
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Granulocyte-macrophage colony stimulating factor is anabolic and interleukin-1beta is catabolic for rat articular chondrocytes.

机译：对于大鼠关节软骨细胞，粒细胞-巨噬细胞集落刺激因子是合成代谢的，白介素-1β是分解代谢的。

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OBJECTIVE: To study the effects of GM-CSF and IL-1beta, both implicated in tissue damage in arthritis, on articular chondrocyte proliferation and metabolism, and to explore their agonist/antagonist effects. METHODS: Chondrocytes were obtained from 1-month-old rats. First-passage monolayers were incubated for 24 h with or without GM-CSF and/or IL-1beta, and labeled with 3H-thymidine, 35S-SO4 and 14C-proline. Proteoglycan and collagen synthesis were analyzed by liquid chromatography and SDS-PAGE. Gene expression was measured by RT-PCR. Results: IL-1beta exerts potent, and GM-CSF weak, inhibitory effects on DNA synthesis. GM-CSF strongly stimulates, and IL-1beta inhibits, proteoglycan and collagen synthesis. IL-1beta suppresses the effect of GM-CSF, and increases the release of radioactive molecules from pre-labeled cartilage fragments; GM-CSF decreases the IL-1beta-induced effect. Interestingly, both cytokines induce the expression of each other's gene. CONCLUSIONS: IL-1beta appears to be a catabolic andanti-anabolic agent for chondrocytes, whereas GM-CSF is mainly anabolic, and blocks the IL-1beta-induced catabolic effect. It is postulated that both agents are implicated in inflammation: IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction.
机译：目的：研究与关节炎组织损伤有关的GM-CSF和IL-1β对关节软骨细胞增殖和代谢的影响，并探讨其激动剂/拮抗剂的作用。方法：从1个月大的大鼠中获得软骨细胞。将第一代单层与或不与GM-CSF和/或IL-1beta一起孵育24小时，并用3H-胸苷，35S-SO4和14C-脯氨酸标记。通过液相色谱和SDS-PAGE分析蛋白聚糖和胶原蛋白的合成。通过RT-PCR测量基因表达。结果：IL-1β对DNA合成具有有效的抑制作用，而GM-CSF具有弱的抑制作用。 GM-CSF强烈刺激蛋白聚糖和胶原蛋白合成，而IL-1β抑制蛋白聚糖和胶原蛋白合成。 IL-1beta抑制GM-CSF的作用，并增加放射性分子从预先标记的软骨片段中的释放; GM-CSF降低IL-1β诱导的作用。有趣的是，两种细胞因子均诱导彼此基因的表达。结论：IL-1β似乎是软骨细胞的分解代谢和抗合成代谢剂，而GM-CSF主要是合成代谢的，并且阻断了IL-1β诱导的分解代谢作用。据推测，这两种药物都与炎症有关：IL-1β促进组织分解代谢和破坏，而GM-CSF促进组织重建。

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