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A laboratory exercise for detecting microbial contaminants

机译:检测微生物污染物的实验室练习

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摘要

The development and evaluation of a 6-hours laboratory class, based on capillary electrophoresis (CE) and the detection of microbial contaminants, is described. It can be easily scaled up or down, to suit class sizes up to 188 and completed in a shorter time scale. CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules. A laboratory exercise has been devised to illustrate how CE-based genetic analysis system processes DNA fragment analysis to detect three microbial contaminants. The protocol is relatively inexpensive and uses standard molecular biology reagents and equipment. (c) 2018 by The International Union of Biochemistry and Molecular Biology, 46(3):279-284, 2018.
机译:描述了基于毛细管电泳(Ce)和检测微生物污染物的6小时实验室类的开发和评价。 它可以很容易地向上或向下扩展,以适应课程尺寸,最多可达188尺寸并以较短的时间尺度完成。 Ce采用窄孔熔融二氧化硅毛细管分离复杂的大和小分子阵列。 已经设计了实验室运动来说明Ce的遗传分析系统如何处理DNA片段分析以检测三种微生物污染物。 该方案相对便宜,并使用标准分子生物学试剂和设备。 (c)2018年由国际生物化学和分子生物学联盟,46(3):279-284,2018。

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  • 作者单位

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

    Hangzhou Dianzi Univ Coll Life Informat Sci &

    Instrument Engn Hangzhou Zhejiang Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
  • 关键词

    Capillary electrophoresis; genetic analysis system; PCR; microbial contaminants;

    机译:毛细管电泳;遗传分析系统;PCR;微生物污染物;

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