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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Lipid-mediated regulation of pore-forming. activity of syringomycin E by thyroid hormones and xanthene dyes
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Lipid-mediated regulation of pore-forming. activity of syringomycin E by thyroid hormones and xanthene dyes

机译：脂质介导的孔径调节。 甲状腺激素和XANTHENE染料的掺量霉素E的活性

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摘要

The effects of dipole modifiers, thyroid hormones (thyroxine and triiodothyronine) and xanthene dyes (Rose Bengal, phloxineB, erythrosin, eosinY and fluorescein) on the pore-forming activity of the lipopeptide syringomycin E (SRE) produced by Pseudomonas syringae were studied in a model bilayer. Thyroxine does not noticeably influence the steady-state number of open SRE channels (N-op), whereas triiodothyronine decreases it 10-fold at - 50mV. Rose Bengal, phloxine B and erythrosin significantly increase Nos by 350, 100 and 70 times, respectively. Eosin Y and fluorescein do not practically affect the pore-forming activity of SRE. Recently, we showed that hormones decrease the dipole potential of lipid bilayers by approximately 60 mV at 50 mu M, while Rose Bengal, phloxine B and erythrosin at 2.5 mu M reduce the membrane dipole potential by 120, 80 and 50 mV, respectively. In the present study using differential scanning microcalorimetry, confocal fluorescence microscopy, the calcein release technique and measurements of membrane curvature elasticity, we show that triiodothyronine strongly affects the fluidity of model membranes: its addition leads to a significant decrease in the temperature and cooperativity of the main phase transition of DPPC, calcein leakage from DOPC vesicles, fluidization of solid domains in DOPC/DPPC liposomes, and promotion of lipid curvature stress. Thyroxine exerts a weaker effect. Xanthene dyes do not influence the phase transition of DPPC. Despite the decrease in the dipole potential, thyroid hormones modulate SRE channels predominantly via the elastic properties of the membrane, whereas the xanthene dyes Rose Bengal, phloxine B and erythrosine affect SRE channels via bilayer electrostatics.
机译：偶极调节剂，甲状腺激素（甲状腺素和三碘甲吡喃酮）和Xanthene染料（玫瑰叶，荧光素，eryhroxin，兴高采烈，荧光和荧光素）的影响对由假单胞菌霉素产生的脂肽霉素E（SRE）的孔形成活性进行了研究模型双层。甲状腺素明显影响稳态开放的SRE通道（N-OP），而三碘罗酮降低10倍 - 50mV。玫瑰叶，氟胺B和赤藓苷分别显着增加350,100和70倍。 eosin Y和荧光素实际上没有影响SRE的孔形成活性。最近，我们展示激素将脂质双层的偶极子潜力在50μm下降约60mV，而玫瑰瓣，氟胺B和2.5μm的赤氧胺素分别将膜偶极电位分别降低120,80和50mV。在本研究中，使用差示扫描微量微量微量测定法，共聚焦荧光显微镜，Calcein释放技术和膜曲率弹性的测量，我们表明三碘罗酮强烈影响模型膜的流动性：其加法导致温度和合作的显着降低DPPC的主相转变，DOPC囊泡的CALCEIN泄漏，DOPC / DPPC脂质体中固体结构域的流化，以及促进脂曲率应力。甲状腺素施加较弱的效果。 XANTHENE染料不会影响DPPC的相转变。尽管偶极电位减少，甲状腺激素主要通过膜的弹性性能调节SRE通道，而XantheNe染料升瓣，氟哌甘肽B和赤藓胺基通过双层静电影响SRE通道。

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