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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >The role of EscD in supporting EscC polymerization in the type III secretion system of enteropathogenic Escherichia coli
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The role of EscD in supporting EscC polymerization in the type III secretion system of enteropathogenic Escherichia coli

机译：ESCD在肠致疗法大肠杆菌III型分泌系统中支持ESCC聚合的作用

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Abstract The type III secretion system (T3SS) is a multi-protein complex that plays a central role in the virulence of many Gram-negative bacterial pathogens. In enteropathogenic Escherichia coli , a prevalent cause of diarrheal diseases, the needle complex base of the T3SS is formed by multi-rings: two concentric inner-membrane rings made by the two oligomerizing proteins (EscD and EscJ), and an outer ring made of a single oligomerizing protein (EscC). Although the oligomerization activity of these proteins is critical for their function and can, therefore, affect the virulence of the pathogen, the mechanisms underlying the oligomerization of these proteins have yet to be identified. In this study, we report that the proteins forming the inner-membrane T3SS rings, EscJ and EscD proteins, are crucial for the oligomerization of EscC. Moreover, we elucidate the oligomerization process of EscD and determine the contribution of individual regions of the protein to its self-oligomerization activity. We show that the oligomerization motif of EscD is located at its N-terminal portion and that its transmembrane domain can self-oligomerize, thus contributing to the self-oligomerization of the full-length EscD. Graphical abstract Display Omitted Highlights ? The oligomerization motif of EscD is located at its N-terminal portion. ? EscD transmembrane domain can self-oligomerize. ? The proteins forming the inner-membrane T3SS rings, EscJ and EscD, are crucial for the oligomerization of EscC.
机译：摘要III型分泌系统（T3SS）是多蛋白复合物，播放在许多革兰氏阴性细菌病原体毒力中心作用。在肠致病性大肠杆菌，腹泻病的一种普遍的原因，该T3SS的针复基是由多环形成：由两个低聚蛋白（ESCD和EscJ），和外环由制成两个同心内膜环单个低聚蛋白（ESCC）。虽然这些蛋白质的低聚活性是他们的功能，可关键，因此，影响了病原体的毒力，这些蛋白质的寡聚化基础的机制仍有待确定。在这项研究中，我们报告，形成内部膜T3SS环蛋白，EscJ和ESCD蛋白质，是食管癌的齐聚至关重要。此外，我们阐明ESCD的低聚合方法，并确定蛋白与其自我齐聚活性的个别地区的贡献。我们表明，ESCD的寡聚基序位于其N-末端部分和其跨膜结构域可以自低聚，从而促进了全长ESCD的自低聚。图形抽象显示省略了亮点？ ESCD的寡聚基序位于其N末端部分。还ESCD跨膜结构域可以自我寡聚化。还形成内 - 膜T3SS环中的蛋白质，EscJ和ESCD，是ESCC的低聚是至关重要的。

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