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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Modification of membrane properties and fatty acids biosynthesis-related genes in Escherichia coli and Staphylococcus aureus : Implications for the antibacterial mechanism of naringenin
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Modification of membrane properties and fatty acids biosynthesis-related genes in Escherichia coli and Staphylococcus aureus : Implications for the antibacterial mechanism of naringenin

机译：在大肠杆菌和金黄色葡萄球菌中改变膜特性和脂肪酸生物合成相关基因：对柚皮素抗菌机制的影响

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Abstract In this work, modifications of cell membrane fluidity, fatty acid composition and fatty acid biosynthesis-associated genes of Escherichia coli ATCC 25922 ( E. coli ) and Staphylococcus aureus ATCC 6538 ( S. aureus ), during growth in the presence of naringenin (NAR), one of the natural antibacterial components in citrus plants, was investigated. Compared to E. coli , the growth of S. aureus was significantly inhibited by NAR in low concentrations. Combination of gas chromatography–mass spectrometry with fluorescence polarization analysis revealed that E. coli and S. aureus cells increased membrane fluidity by altering the composition of membrane fatty acids after exposure to NAR. For example, E. coli cells produced more unsaturated fatty acids (from 18.5% to 43.3%) at the expense of both cyclopropane and saturated fatty acids after growth in the concentrations of NAR from 0 to 2.20mM. For S. aureus grown with NAR at 0 to 1.47mM, the relative proportions of anteiso-branched chain fatty acids increased from 37.2% to 54.4%, whereas iso-branched and straight chain fatty acids decreased from 30.0% and 33.1% to 21.6% and 23.7%, respectively. Real time q-PCR analysis showed that NAR at higher concentrations induced a significant down-regulation of fatty acid biosynthesis-associated genes in the bacteria, with the exception of an increased expression of fabA gene. The minimum inhibitory concentration (MIC) of NAR against these two bacteria was determined, and both of bacteria underwent morphological changes after exposure to 1.0 and 2.0 MIC. Graphical abstract Display Omitted Highlights ? The growth of S. aureus was significantly inhibited in low concentrations of NAR. ? E. coli cells showed to be less sensitive to NAR than S. aureus . ? Exposing to NAR changed the membrane fluidity and fatty acid composition. ? SEM revealed that morphological changes of bacterial cells induced by NAR.
机译：摘要在这项工作中，在Naringenin的生长期间，在柚皮蛋白（ NAR），研究了柑橘植物中的天然抗菌组分之一。与大肠杆菌相比，NAR在低浓度下显着抑制了S.UUREUS的生长。气相色谱 - 质谱与荧光偏振分析的组合显示，通过在暴露于NAR后改变膜脂肪酸的组成来增加膜流动性。例如，大肠杆菌细胞以浓度为0至2.20mm的浓度在浓度为0至2.20mm的浓度后产生更多不饱和脂肪酸（从18.5％至43.3％）以牺牲环丙烷和饱和脂肪酸。对于用NAR以0至1.47mm生长的金黄色葡萄球菌，脱离链脂肪酸的相对比例从37.2％增加到54.4％，而ISO-支链和直链脂肪酸从30.0％降低，33.1％至21.6％分别为23.7％。实时Q-PCR分析表明，较高浓度下的NAR诱导细菌中脂肪酸生物合成相关基因的显着下调，除了Faba基因的表达增加。测定对这两种细菌的​​NAR的最小抑制浓度（MIC），并且细菌在暴露于1.0和2.0麦克风后经过形态变化。图形抽象显示省略了亮点？在低浓度的NAR中显着抑制了S.UUREUS的生长。还大肠杆菌细胞显示对NAR比金黄色葡萄球菌不太敏感。还曝光肿瘤改变了膜流动性和脂肪酸组成。还SEM显示NAR诱导的细菌细胞的形态变化。

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