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首页> 外文期刊>Bioanalysis >Simultaneous quantification of high-dose naloxone and naloxone-3--d-glucuronide in human plasma by UHPLC-MS/MS
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Simultaneous quantification of high-dose naloxone and naloxone-3--d-glucuronide in human plasma by UHPLC-MS/MS

机译：通过UHPLC-MS / MS在人血浆中同时定量高剂量纳洛酮和Naloxone-3 - D-葡糖醛醛酮

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Aim: High-dose administration of the -opioid receptor inverse agonist naloxone (NX), has previously been demonstrated to reinstate nocifensive behavior in the late phase of inflammatory injuries. However, no current analytical methods can provide pharmacokinetic insight into the pharmacodynamic response of high-dose administration of NX. Materials & methods:Based on protein precipitation using 50l human plasma, NX and naloxone--d-glucuronide (NXG) was analysed by UHPLC-MS/MS with 6 min cycle time.Results: A method for quantification of high-dose administered NX and NXG was developed and validated with intra- and interday precision and accuracy within 8.5% relative standard deviation (RSD)and -1.2-5.5% relative error (RE) for NX and 9.6% RSD and 0.6-6.5% RE for NXG. The method show excellent internal standard corrected matrix effects. Conclusion: A rapid UHPLC-MS/MS method was developed for quantification of NX and NXG in human plasma within 10-4000ng/ml.
机译：目的：先前已经证明了高剂量给予 - 己酯受体反向激动剂纳尔诺酮（NX），以恢复炎症损伤后期的滴度。 然而，没有目前的分析方法可以为高剂量施用NX的药代动力学洞察力提供药代动力学洞察。 材料和方法：基于使用50L人血浆的蛋白质沉淀，通过UHPLC-MS / MS分析NX和NALOXONE - D-葡糖醛酸酯（NXG），具有6分钟的循环时间。结果：一种用于定量高剂量施用NX的方法 在8.5％的相对标准偏差（RSD）和-1.2-5.5％的相对误差（RE）以内开发并验证了NX和9.6％RSD的相对误差（RE）和NXG。 该方法显示出优异的内标校正矩阵效应。 结论：在10-4000ng / ml内，开发了一种快速的UHPLC-MS / MS方法以在人血浆中定量NX和NXG。

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