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首页> 外文期刊>Cytometry, Part A: the journal of the International Society for Analytical Cytology >In vivo photoacoustic flow cytometry for early malaria diagnosis
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In vivo photoacoustic flow cytometry for early malaria diagnosis

机译：体内光声流式细胞术用于早期疟疾诊断

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摘要

In vivo photoacoustic (PA) flow cytometry (PAFC) has already demonstrated a great potential for the diagnosis of deadly diseases through ultrasensitive detection of rare disease-associated circulating markers in whole blood volume. Here, we demonstrate the first application of this powerful technique for early diagnosis of malaria through label-free detection of malaria parasite-produced hemozoin in infected red blood cells (iRBCs) as high-contrast PA agent. The existing malaria tests using blood smears can detect the disease at 0.001-0.1% of parasitemia. On the contrary, linear PAFC showed a potential for noninvasive malaria diagnosis at an extremely low level of parasitemia of 0.0000001%, which is approximate to 10(3) times better than the existing tests. Multicolor time-of-flight PAFC with high-pulse repetition rate lasers at wavelengths of 532, 671, and 820 nm demonstrated rapid spectral and spatial identification and quantitative enumeration of individual iRBCs. Integration of PAFC with fluorescence flow cytometry (FFC) provided real-time simultaneous detection of single iRBCs and parasites expressing green fluorescence proteins, respectively. A combination of linear and nonlinear nanobubble-based multicolor PAFC showed capability to real-time control therapy efficiency by counting of iRBCs before, during, and after treatment. Our results suggest that high-sensitivity, high-resolution ultrafast PAFC-FFC platform represents a powerful research tool to provide the insight on malaria progression through dynamic study of parasite-cell interactions directly in bloodstream, whereas portable hand-worn PAFC device could be broadly used in humans for early malaria diagnosis. (c) 2016 International Society for Advancement of Cytometry
机译：体内光声（PA）流式细胞仪（PAFC）已通过超灵敏检测全血量中与疾病相关的罕见循环标志物，证明了在致命疾病诊断方面的巨大潜力。在这里，我们通过无标签检测疟疾寄生虫产生的作为高对比度PA药剂的红细胞（iRBCs）中的疟原虫产生的血红蛋白，证明了这项功能强大的技术在疟疾早期诊断中的首次应用。现有的使用血液涂片的疟疾测试可以在0.001-0.1％的寄生虫病中检测出该疾病。相反，线性PAFC在0.0000001％的极低寄生虫水平下显示了非侵入性疟疾诊断的潜力，这比现有测试高约10（3）倍。多色飞行时间PAFC与高脉冲重复率激光器在532、671和820 nm的波长下显示出快速的光谱和空间识别以及单个iRBC的定量计数。 PAFC与荧光流式细胞仪（FFC）的集成分别提供了实时同时检测单个iRBC和表达绿色荧光蛋白的寄生虫的功能。线性和非线性的基于纳米气泡的多色PAFC的结合显示了通过计数治疗前后，治疗过程中和治疗后iRBC的实时控制治疗效率的能力。我们的研究结果表明，高灵敏度，高分辨率的超快PAFC-FFC平台代表了一个强大的研究工具，可通过直接在血液中动态研究寄生虫-细胞相互作用来提供有关疟疾进展的见识，而便携式手持式PAFC装置可能会广泛用于人类早期疟疾诊断。 （c）2016国际细胞计数学会

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