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首页> 外文期刊>Bulletin of the European Association of Fish Pathologists >In vitro adhesion and infectiveness of Piscirickettsia salmonis on mussel shells Mytilus chilensis
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In vitro adhesion and infectiveness of Piscirickettsia salmonis on mussel shells Mytilus chilensis

机译：在贻贝壳上的Piscirickettsia salmonis的体外附着力和感染性

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摘要

The salmon industry in Chile is seriously affected by piscirickettsiosis or rickettsial salmonid septicemia (SRS) that produces annual losses of over 300 million dollars. Its etiological agent Piscir-ickettsia salmonis is an intracellular facultativepathogen that was initially isolated in Chile and has been reported from other salmon-producing countries. It has been demonstrated in vitro that the microorganism attaches to the eggs through the so-called Piscirickettsial Attachment Complex. In the present work, we studied the ability of P. salmonis to bind to the surface of mussel shells (Mytilus chilensis), an endemic bivalve blue mussel from southern Chile that is cultivated in the same areas where salmon is kept. The bacterium was cultured in theCHSE-2f 4 cell line free of antibiotics and fungistats until a 100% cytopathic effect (CPE) was produced. To study attachment, pieces of mussel shells of approximately 0.5 cm2 that had been subjected to a cleaning and disinfection process were placed ina bacterial suspension for 1, 24, or 48 h at 15 °C, fixed and processed for scanning electron microscopy. Additionally, two pieces of shells subjected to 48 h of exposure were rinsed in sterile PBS buffer (pH = 7.2) and placed in a culture bottle with aconfluent CHSE-214 cell monolayer, where they yielded CPE around the edge starting three days after infection to eventually producing 100% CPE after 14 days of incubation. The results demonstrate adhesion to and subsequent release of infective P. salmonis from the surface of mussel shells, a phenomenon that may have epidemiological implications since P. salmom's-exposed mollusks may serve as reservoirs and/or vectors of the agent.
机译：智利的鲑鱼产业受到氏锡克利克特病的严重影响，或唾液酸血清败血症（SRS），产生超过3亿美元的年度亏损。其病因症Piscir-ickettsia Salmonis是一种智利最初分离的细胞内致力学性，并且已从其他鲑鱼的国家报告。已经在体外证明了微生物通过所谓的皮鱼柱附着络合物附着在卵中。在目前的工作中，我们研究了P. Salmonis与贻贝壳（Mytilus Chilensis）结合的能力，来自智利南部的人群双向蓝贻贝，在鲑鱼的同一区域栽培。在没有抗生素和真菌的Thechse-2F 4细胞系中培养细菌，直至产生100％细胞病变效应（CPE）。为了研究附着的附件，将已进行清洁和消毒过程的大约0.5cm 2的贻贝壳在15℃下置于1,24或48小时，固定并加工用于扫描电子显微镜。另外，在无菌PBS缓冲液（pH = 7.2）中冲洗两片暴露于48小时的壳，并置于带有Aconfluent CHSE-214细胞单层的培养瓶中，在感染后三天开始围绕边缘的CPE。最终在孵化14天后产生100％的CPE。结果表明，从贻贝壳表面释放的粘附和随后释放来自贻贝壳表面的现象，其可能具有流行病学意义，因为P. Salmom的暴露的软体动物可以用作药剂的储层和/或载体。

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    J. Larenas; M. Perez; C. Moraleda;

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    Department of Animal Pathology Faculty of Veterinary and Animal Sciences University of Chile Santa Rosa 11735 La Pintana Santiago Chile;

    Department of Animal Pathology Faculty of Veterinary and Animal Sciences University of Chile Santa Rosa 11735 La Pintana Santiago Chile;

    Department of Animal Pathology Faculty of Veterinary and Animal Sciences University of Chile Santa Rosa 11735 La Pintana Santiago Chile;

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  • 正文语种 eng
  • 中图分类 水产、渔业;
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