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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Silencing non-SMC chromosome-associated polypeptide G inhibits proliferation and induces apoptosis in hepatocellular carcinoma cells
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Silencing non-SMC chromosome-associated polypeptide G inhibits proliferation and induces apoptosis in hepatocellular carcinoma cells

机译：沉默的非SMC染色体相关的多肽G抑制肝细胞癌细胞中的增殖和诱导细胞凋亡

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摘要

The present study was designed to investigate the significance of non-structural maintenance of chromosomes (non-SMC) chromosome-associated polypeptide G (NCAPG), a subunit of condensin complex I, in the development of hepatocellular carcinoma (HCC). NCAPG protein expression in human HCC and paracancerous hepatic tissues were examined using immunohistochemistry, and NCAPG mRNA expression in HCC cell lines were quantified using quantitative RT-PCR. Lentivirus-mediated RNA interference was used to silence NCAPG in HCC cells. Cell proliferation was monitored by MTT assay. Cell colony-forming capacity was measured by colony formation assay. Apoptosis was determined by flow cytometry. The results showed that increased protein expression of NCAPG was found in HCC tissues compared with the matched paracancerous hepatic tissues. At the mRNA level, increased expression of NCAPG was found in HCC cells as opposed to the normal hepatocytes. Silencing of NCAPG in BEL-7404 and SMMC-7721 cells led to decreased cell proliferation and increased apoptosis. These changes were associated with increased mRNA expressions of P53, P27, and Bad, but decreased mRNA expression of EGFR, Akt, survivin, and JNK. NCAPG might play an oncogenic role in the development of liver cancer. Further studies to clarify its role and underlying mechanisms in the development of liver cancer are warranted.
机译：本研究旨在探讨染色体（非SMC）染色体相关多肽G（NCAPG），凝聚络合物I的亚基的非结构维持的重要性，在肝细胞癌（HCC）的发育中。使用免疫组织化学检查人HCC和副肝癌组织中的NCAPG蛋白表达，并使用定量RT-PCR定量HCC细胞系中的NCAPG mRNA表达。慢病毒介导的RNA干扰用于沉默HCC细胞中的NCAPG。通过MTT测定监测细胞增殖。通过菌落形成测定法测量细胞核性形成能力。细胞凋亡由流式细胞术确定。结果表明，与匹配的副血管肝组织相比，在HCC组织中发现了NCAPG的蛋白表达增加。在mRNA水平下，在HCC细胞中发现NCAPG的增加表达，而不是正常肝细胞。 BEL-7404和SMMC-7721细胞中NCAPG的沉默导致细胞增殖降低和凋亡增加。这些变化与p53，p27和坏的mRNA表达增加相关，但EGFR，AKT，Survivin和JNK的mRNA表达减少。 NCAPG可能在肝癌的发展中发挥致癌作用。进一步的研究，以澄清其作用和潜在机制在肝癌的发育中受到必要。

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